STUDY IN CYTOTOXICITY OF GENTAMYCIN TO CORNEAL EPITHELIUM AND ENDOTHELIUM IN TISSUE CULTURE  

作　　者：Ning Lin Xiangming Gong Quanjin Xie Miaorong Shao Eye Research Institute, Zhongshan Ophthalmic Center Sun Yat-sen University of Medical Sciences Guangzhou, China 

出　　处：《Eye Science》1989年第Z1期32-35,共4页眼科学报（英文版）

摘　　要：A study in cytotoxicity of gentamyein to tissue-cultured bovine corneal endothelial cells and rabbit corneal epithelial cells is reported. When the cultured cells reached confluence, they were exposed to tissue culture media containing gentamycin for 6 hours. We founl that 0.5% gentamycin caused significant damage to corneal epithelial cells---diffuse plasmolysis, with scattered cell necrosis and 5% loss.While corneal endothelial cells were exposed to 1.6 mg/ml gentamycin, extensive cell loss (approximate- ly 15%) was observed. The damaged cells recovered their normal morphology after 24 hours. When the concentration of gentamycin increased twice, serious damage to cells occured. The area of cell loss reached 40%, and the recovery of cellular morphology Was much slower. This study demonstrates that gentamycin potential cytotoxicity to corneal epithelium and endothelium, suggesting that gentamycin should be rationally used in the treatment of ocular diseases.A study in cytotoxicity of gentamyein to tissue-cultured bovine corneal endothelial cells and rabbit corneal epithelial cells is reported. When the cultured cells reached confluence, they were exposed to tissue culture media containing gentamycin for 6 hours. We founl that 0.5% gentamycin caused significant damage to corneal epithelial cells---diffuse plasmolysis, with scattered cell necrosis and 5% loss.While corneal endothelial cells were exposed to 1.6 mg/ml gentamycin, extensive cell loss (approximate- ly 15%) was observed. The damaged cells recovered their normal morphology after 24 hours. When the concentration of gentamycin increased twice, serious damage to cells occured. The area of cell loss reached 40%, and the recovery of cellular morphology Was much slower. This study demonstrates that gentamycin potential cytotoxicity to corneal epithelium and endothelium, suggesting that gentamycin should be rationally used in the treatment of ocular diseases.

关 键 词：ENDOTHELIUM CORNEAL EPITHELIUM rabbit SLOWER exposed bovine twice cultured scattered 

分 类 号：R77[医药卫生—眼科]