从黑曲霉发酵粉中分离纯化一种内切β-葡聚糖苷酶  

Isolation and purification of an endoglucanase from Aspergillus niger

作　　者：张志强 郭春腾[1] 林佳任 饶平凡[1] 

机构地区：[1]福州大学生物工程研究所,福建福州350002 [2]贵州老来福药业有限公司,贵州贵阳550003

出　　处：《福州大学学报（自然科学版）》2002年第5期636-640,共5页Journal of Fuzhou University(Natural Science Edition)

摘　　要：通过硫酸铵分级沉淀 ,CM -SephadexC - 2 5 ,DEAE -SephadexA - 5 0 ,POROS 2 0HQ离子交换色谱分离方法 ,从黑曲霉发酵粉中分离纯化了一种新的内切 β -葡聚糖苷酶 .该酶在还原条件下分子量为 39.2kD ,最适pH为 4.0 ,最适温度为 5 5℃ ,Km 为 7.41mg mL .A novel cellulase was isolated and purified to homogeneity from a commercial Aspergillus niger cellulase preparation by a combination of ammonium sulfate and three steps of ion exchange chromatograph of CM-Sephadex C-25, DEAE-Sephadex A-50 and POROS 20 HQ. The molecular weight of the purified enzyme is 39.2?kD under reduced conditions by SDS-PAGE; the optimal pH of the enzyme is 4.0, and the optimal temperature is 55?℃, its K m is 7.41?mg/mL.

关 键 词：发酵粉 黑曲霉 内切Β-葡聚糖苷酶 分离 纯化 动力学性质 纤维素酶 

分 类 号：TQ925.9[轻工技术与工程—发酵工程]