Effect of astragaloside Ⅳ on T, B lymphocyte proliferation and peritoneal macrophage function in mice  

黄芪皂甙Ⅳ对小鼠T、B淋巴细胞增殖和腹腔巨噬细胞功能的影响(英文)

作　　者：王燕平[1] 李晓玉[1] 宋纯青 胡之璧[2] 

机构地区：[1]中国科学院上海生命科学研究院上海药物研究所,上海200031 [2]上海中医药大学,上海中国200032

出　　处：《Acta Pharmacologica Sinica》2002年第3期75-78,共4页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China, № 98pd09.

摘　　要：To investigate the effect of astragaloside Ⅳ (ASI) on T, B lymphocyte proliferation, antibody production, and cytokines produced by murine peritoneal macro-phages. METHODS: MTT assay was used to determine T, B lymphocyte proliferation and quantitative hemolysin spectrophotometry (QHS) assay was applied to test antibody production; IL-1 production was measured by thymocyte proliferation assay; TNF-α production was determined by the cytotoxicity assay against L929 cells. RESULTS: 1) In vivo, ASI 50 - 200 mg/kg ig for 7 d increased T lymphocyte proliferation and antibody production, and ASI .50 - 100 mg/kg ig for 7 d increased B lymphocyte proliferation but ASI 200 mg/kg had no effect on B lymphocyte proliferation; 2) In vitro, ASI increased T, B lymphocyte proliferation only at 100 nmol/L; 3) ASI increased IL-1 activity at 1 nmol/L in vitro, but decreased it at 100 and 1000 nmol/L; 4) ASI inhibited TNF-α activity with or without LPS-stimulation in vitro. CONCLUSION: ASI increased T, B lymphocyte proliferation and antibody production in vivo and in vitro; but inhibited productions of IL-1 and TNF-α from peritoneal macrophages in vitro.目的:探讨黄芪皂苷Ⅳ(ASI)对小鼠T、B淋巴细胞增殖和腹腔巨噬细胞分泌的细胞因子的影响.方法:采用MTT法检测T、B淋巴细胞的增殖;采用分光光度计测定法检测抗体活性;IL-1活性用胸腺细胞增殖法测定;TNF-α活性用L929细胞杀伤法测定.结果:1)ASI50-200mg/kg ig 7天能够促进T淋巴细胞增殖和抗体生成,而ASI 50-100mg/kg能够促进B淋巴细胞增殖,但是220mg/kg对B淋巴细胞增殖无影响;2)ASI体外仅在100nmol/L对T、B淋巴细胞有促进作用;3)ASI 1 nmol/L可以促进腹腔巨噬细胞分泌IL-1,而100-1000nmol/L则抑制腹腔巨噬细胞分泌IL-1;4)ASI体外可以抑制LPS刺激或无LPS刺激下的腹腔巨噬细胞分泌TNF-α.结论:ASI能够促进小鼠T、B淋巴细胞的增殖和抗体生成,同时可以抑制腹腔巨噬细胞体外分泌IL-1和TNF-α.

关 键 词：Astragalus membranaceus SAPONINS T-LYMPHOCYTES B-LYMPHOCYTES antibody formation peritoneal macrophages INTERLEUKIN-1 tumor necrosis factor 

分 类 号：R285[医药卫生—中药学]