Retrovirus-mediated herpes simplex virus thymidine kinase gene therapy approach for hepatocellular carcinoma  

作　　者：GAODINGCHENG WEIAN 等 

机构地区：[1]DepartmentofCellBiology,CapitalUniversityofMedicalSciences100054Beijing,China [2]Correspondence：AnWeiM.D

出　　处：《Cell Research》1999年第3期225-235,共11页细胞研究（英文版）

摘　　要：The therapeutic effect of herpes simplex virus thymidine kinase/ganciclovir (HSV-tk/GCV) system on hepatocellular carcinoma was studied in this experiment. The tk-containing retroviral recombinants were used to infect hepatoma cells (BEL-7402) and the cells were treated with ganciclovir (0-1000 microg/ml). The results showed that HSV-tk gene could be efficiently transferred in vitro into hepatoma cells and stably expressed. The growth potential of the tk-containing cells was significantly inhibited by GCV (P

关 键 词：Gene Therapy Animals Blotting  Southern Carcinoma  Hepatocellular Cell Death GANCICLOVIR Gene Expression HETEROCHROMATIN Humans Liver Neoplasms Male MICE Mice  Inbred BALB C Mice  Nude Microscopy  Electron Research Support  Non-U.S. Gov't RETROVIRIDAE Simplexvirus Thymidine Kinase Transfection Tumor Cells  Cultured 

分 类 号：R735.7[医药卫生—肿瘤] R730.5[医药卫生—临床医学]