金枪鱼胰脏酶解多肽对db/db糖尿病小鼠肾脏的保护作用  

Protective Effect of Enzymatic Hydrolysate of Tuna Pancreatic Proteins on Kidney in db/db Diabetic Mice

作　　者：孙婷婷[1] 李妍妍[2] 周君[1] 张迪骏[1] 李晔[1] 张春丹[1] 何珊[1] 黄忠白 苏秀榕[1] SUN Tingting;LI Yanyan;ZHOU Jun;ZHANG Dijun;LI Ye;ZHANG Chundan;HE Shan;HUANG Zhongbai;SU Xiurong(School of Marine Sciences, Ningbo University, Ningbo 315211, China;College of Agriculture and Life Sciences, Cornell University, Ithaca 14850, USA)

机构地区：[1]宁波大学海洋学院,浙江宁波315211 [2]康奈尔大学农业与生命科学学院,美国纽约州伊萨卡14850

出　　处：《食品科学》2017年第3期182-187,共6页Food Science

基　　金：海洋经济创新发展区域示范项目(2013710);宁波市科技局农业与社发重大科技项目(2010C10040);宁波市教育局重点学科资助项目

摘　　要：目的:研究金枪鱼胰脏酶解多肽对db/db糖尿病小鼠体质量、血清胰岛素、糖化血红蛋白、血脂的影响,为进一步开发金枪鱼胰脏、研制新型保健型食品提供理论依据。方法:雄性Db/m小鼠为空白对照组,雄性db/db小鼠30只,分为模型对照组、阳性药组(甲基巴多索隆9.75 mg/(kg·d))、实验组,实验组灌胃金枪鱼胰脏酶解多肽(50 mg/(kg·d))。10周后测定各组小鼠体质量、血清胰岛素、糖化血红蛋白、血脂水平等指标。利用基因芯片技术,比较不同组间肾脏基因的表达水平。结果:与模型对照组相比,实验组能显著降低糖尿病小鼠的体质量、血清胰岛素、糖基化血红蛋白水平;降低总胆固醇、甘油三酯、低密度脂蛋白胆固醇水平,升高高密度脂蛋白胆固醇水平。并且,与模型对照组的差异表达比较,实验组基因表达水平有显著改变,获得差异基因678个,其中161个上调,517个下调。结论:金枪鱼胰脏酶解多肽可以改善db/db糖尿病小鼠的体质量、血清胰岛素、糖化血红蛋白、血脂水平,其作用效应可能与其下调醛固酮合成相关基因CYP11B1的表达水平有关。The present study aimed to evaluate the effect of peptides produced by enzymatic hydrolysis of tuna pancreatic proteins on body weight, fasting serum insulin (FINS), glycated hemoglobin (HbA1c), and serum lipid levels of db/db diabetic mice for the purpose of providing theoretic evidence for the development of new healthy foods from tuna pancreas.Methods: Ten male Db/m mice were used as blank control group. A total of 30 male leptin gene knockout db/db mice were divided into model control group, positive drug control group and experimental group. The mice in the experimental group were gavaged with the peptides derived from tuna pancreatic proteins. After 10 weeks, the body weight, FINS, HbA1c,and serum lipid biochemical indexes in all groups of mice were examined. The gene expression levels in the kidney were evaluated using gene chip technology. Results: In comparison with the control group, the experimental group demonstrated a significant decrease in body weight, FINS, HbA1c, serum total cholesterol, triglyceride and low-density lipoprotein cholesterol levels and an obvious increase in high-density lipoproteincholesterol. The gene expression levels in experimental group were also significantly different from those in the control group. A total of 678 differentially expressed genes were found, 161 of which were up-regulated and 517 of which were down-regulated. Conclusion: The peptides derived from tuna pancreatic proteins by enzymatic hydrolysis can effectively control body weight, FINS, HbA1c and serum lipid levels in db/db diabetic mice, likely by decreasing the expression of aldosterone synthase gene (CYP11B1).

关 键 词：金枪鱼胰脏 酶解多肽 糖尿病 DB/DB小鼠 CYP11B1 

分 类 号：TS201.4[轻工技术与工程—食品科学]