植物乳杆菌FS5-5在盐胁迫下的转录组学分析  

Transcriptomic Analyses of Lactobacillus plantarum FS5-5 against Salt Stress

作　　者：宋雪飞[1] 郭晶晶[1] 姜静[1] 唐筱扬 张颖[1] 乌日娜[1,2] SONG Xuefei;GUO Jingjing;JIANG Jing;TANG Xiaoyang;ZHANG Ying;WU Rina(College of Food Science, Shenyang Agricultural University, Shenyang 110866, China;State Key Laboratory of Food Science and Technology, College of Food Science, Jiangnan University, Wuxi 214122, China)

机构地区：[1]沈阳农业大学食品学院,辽宁沈阳110866 [2]江南大学食品学院,食品科学与技术国家重点实验室,江苏无锡214122

出　　处：《食品科学》2017年第6期20-26,共7页Food Science

基　　金：国家自然科学基金青年科学基金项目(31000805);国家自然科学基金面上项目(31471713);中国博士后科学基金项目(2014M560395);辽宁省农业领域青年科技创新人才培养计划项目(2014048);辽宁省高等学校优秀人才支持计划项目(LR2015059);江苏省博士后科研资助计划项目(1402071C)

摘　　要：以分离自东北自然发酵大酱中的一株耐盐植物乳杆菌FS5-5(Lactobacillus plantarum FS5-5)为实验对象,在转录水平上对该菌株盐胁迫相关基因表达进行了研究。结果表明:在对数生长期,表达显著下调的共29个基因,分别为参与碳水化合物转运和代谢的4个基因,氨基酸转运和代谢的9个基因,维生素代谢的3个基因,核苷酸代谢的6个基因,遗传信息翻译、核糖体结构和形成的7个基因;表达显著上调的有参与碳水化合物转运和代谢的4个基因。这些变化可能与L.plantarum FS5-5的盐胁迫机制密切相关。选取参与维生素代谢的3个基因和核苷酸代谢的1个基因,通过实时定量聚合酶链式反应对转录组学结果进行验证,结果表明两种方法中基因表达趋势一致。实验对L.plantarum FS5-5的盐胁迫反应进行了比较全面的分析,为提高工业生产中菌株的耐受性提供了理论依据。This investigation studied the expression of salt stress-related genes in Lactobacillus plantarum FS5-5,asalt-tolerance strain isolated from naturally fermented miso in northeastern China,at the level of transcription.Resultsshowed that in the logarithmic growth phase,29genes were significantly down-regulated,including four ones involved incarbohydrate transport and metabolism,nine ones involved in amino acids transport and metabolism,three ones involved invitamin metabolism,six ones involved in nucleotide metabolism and seven ones involved in genetic information translation,ribosomal structure and biogenesis.Four genes involved in carbohydrate transport and metabolism were significantly upregulated,which might be closely associated with salt stress resistance of L.plantarum FS5-5.Three genes involved invitamin metabolism and one gene involved in nucleotide metabolism were selected to be analyzed by real-time fluorescentquantitative polymerase chain reaction and the results showed that the expression of these genes exhibited the same trend in thesetwo methods.The comprehensive analysis of the mechanism of action of L.plantarum FS5-5to resist salt stress presented in thisstudy which would provide a theoretical basis to improve the tolerance of strains in industrial production.

关 键 词：植物乳杆菌 盐胁迫 实时定量聚合酶链式反应 转录组学 

分 类 号：Q939.9[生物学—微生物学]