毕赤酵母热致死动力学模型的建立与检验  

Construction and Verification of a Model for the Lethal Thermal Kinetics of Pichia pastoris

作　　者：田康明[1] 段威 王正祥[1] 路福平[2] TIAN Kangming;DUAN Wei;WANG Zhengxiang;LU Fuping(College of Chemical Engineering and Materials Science,Tianjin University of Science & Technology,Tianjin 300457,China;Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,College of Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China)

机构地区：[1]天津科技大学化工与材料学院,天津300457 [2]工业发酵微生物教育部重点实验室天津科技大学生物工程学院,天津300457

出　　处：《天津科技大学学报》2017年第2期24-29,共6页Journal of Tianjin University of Science & Technology

摘　　要：毕赤酵母作为最成熟的蛋白质过量表达平台微生物之一,广泛应用于酶制剂、功能性大分子等大宗产品的发酵制造.建立毕赤酵母常规温度下(50~60,℃)的热致死动力学模型,有助于指导毕赤酵母发酵制造体系的菌体后处理工艺,增加菌体利用价值.为了建立毕赤酵母在50~60,℃条件下热致死动力学模型,将不同菌体量的毕赤酵母菌液在50、55、60,℃条件下进行不同时间热灭活处理,通过平板培养活细胞计数的方法评价不同处理方式下毕赤酵母的热致死效果,得到的致死曲线用Weibull模型和多元回归方程进行拟合构建热致死动力学模型,并对建立的模型进行数学检验和实用性检验.结果表明:建立的毕赤酵母热致死动力学模型可靠,具有实际使用价值.本研究所建立的模型能较好地模拟不同温度、不同处理时间对不同菌体量的毕赤酵母热致死效果的影响.并可以为毕赤酵母热灭活处理条件的确定提供计算依据.Pichia pastoris,as one of the most mature protein overexpression platforms in the study of microorganisms,waswidely used for bulk product manufacturing such as enzyme and functional macromolecule.Establishing a lethal thermalkinetics model of Pichia pastoris under various heat treatment conditions from50,℃to60,℃will supply a guide to Pichiapastoris cell manufacturing system and increase the utilization value of cellmass.The lethal thermal kinetics of Pichia pastorisunder various heat treatment conditions from50,℃to60,℃was investigated,and the availability and feasibility of themodel was verified by live cell lethal tests.The cell culture of Pichia pastoris GS115with different optical density was incubatedat50,℃,55,℃and60,℃for various heating time respectively,then the obtained lethal curves were fitted using theWeibull model and calculated using multiple regression equation to establish the lethal thermal kinetics model.Subsequentlythe feasibility and pratical lethality were confirmed by live cell lethal tests.A Pichia pastoris lethal thermal kinetics modelwas thus established,and its availability and feasibility were confirmed.The established model in this study can be used todescribe the lethal thermal kinetics of Pichia pastoris under various heat treatment conditions.The methods constructed herecan be used for getting the suitable heat treatment process for heat inactivation of Pichia pastoris.

关 键 词：毕赤酵母 致死动力学 预测微生物学 Weibull模型 

分 类 号：Q939.97[生物学—微生物学]