改良QuEChERS技术结合液相色谱-串联质谱联用法同时快速检测辣椒制品中14种非法添加工业染料  

Rapid Simultaneous Determination of 14 Adulterated Industrial Dyes in Chilli Products by a Modified QuEChERS Method Coupled with LC-MS/MS

作　　者：陈林 温家欣[2] 吴霞[3] 雷毅[2,4] 张荣[4] CHEN Lin;WEN Jiaxin;WU Xia;LEI Yi;ZHANG Rong(Nanyang Institute for Food and Drug Control, Nanyang 473059, China;Guangdong Institute for Drug Control,Guangzhou 510180, China;Agilent Technologies Co. Ltd., Guangzhou 510613, China;School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China)

机构地区：[1]南阳市食品药品检验所,河南南阳473059 [2]广东省药品检验所,广东广州510180 [3]安捷伦科技有限公司,广东广州510613 [4]广东药科大学药科学院,广东广州510006

出　　处：《食品科学》2017年第8期296-302,共7页Food Science

基　　金：广东省省级科技计划项目(2013B090200059);广州市健康产品非法添加化学成分快速检测技术重点实验室项目(2013年);广东省食品药品检验检测技术创新专项(2015ZX01)

摘　　要：采用改良Qu ECh ERS技术作为前处理方法,建立了辣椒制品中14种非法添加工业染料的液相色谱-串联质谱联用快速检测方法。样品采用乙腈-丙酮(7∶3,V/V)提取,经EMR-Lipid净化,以Agilent Poroshell 120 EC C18(50 mm×2.1 mm,2.7μm)色谱柱分离,采用多反应监测模式检测,外标法定量分析。14种成分在11 min内完成分离,检出限为0.4~7.1μg/kg,不同加标水平的平均回收率为70.5%~102.1%,相对标准偏差为0.3%~9.3%。本方法准确、快速、重复性好,可为辣椒制品中非法添加工业染料的检测提供一种更加快速、简便的技术支持。A rapid method for the simultaneous determination of14adulterated industrial dyes in chilli products was developed by using liquid chromatography-tandem mass spectrometry(LC-MS/MS)based on a modified quick,easy,cheap,effective,rugged,and safe(QuEChERS)sample pretreatment method.Samples were extracted with acetonitrile-acetone(7:3,V/V),and then the extract was cleaned up using Enhanced Matrix Removal Lipid(EMR-Lipid).The chromatographic separation was performed on an Agilent Poroshell120EC C18(50mm×2.1mm,2.7μm)column.The separated analytes were analyzed with LC-MS/MS by multiple reaction monitoring(MRM),and quantified by the external standard method.The results showed that the14pigments were separated completely in11minutes,and their quantitative detection limits ranged from0.4to7.1μg/kg.The average recoveries at three spiked levels ranged from70.5%to102.1%with relative standard deviations(RSDs)(n=6)of0.3%-9.3%.The method is quick,accurate,and sensitive,and can be applied to the determination of14adulterated industrial dyes in chilli products.

关 键 词：QUECHERS EMR-Lipid 液相色谱-串联质谱联用 辣椒制品 非法添加工业染料 

分 类 号：O658[理学—分析化学]