狐狸和貉子动物源性成分双重PCR鉴别  

Duplex PCR Distinction of Food Ingredients Derived from Alopex lagopus and Nyctereutes procyonoides

作　　者：杜利强[1] 章晶晶[2] 张涛[2] 齐艳玲[1] 李顺才[1] 李岩[2] 周巍[2] 张岩[2] DU Liqiang;ZHANG Jingjing;ZHANG Tao;QI Yanling;LI Shuncai;LI Yan;ZHOU Wei;ZHANG Yan(Life Science and Technology Institute, Hebei Normal University of Science and Technology, Changli 066604, China;Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang 050071, China)

机构地区：[1]河北科技师范学院生命科技学院,河北昌黎066604 [2]河北省食品检验研究院,河北省食品安全重点实验室,河北石家庄050071

出　　处：《食品科学》2017年第8期303-307,共5页Food Science

基　　金：河北省科技厅重点研发计划项目(16275502D);河北省高等学校科学技术研究青年基金项目(QN2014159)

摘　　要：根据狐狸和貉子线粒体基因中保守序列设计特异性引物,建立一种基于双重聚合酶链式反应(polymerase chain reaction,PCR)的肉及肉制品狐狸和貉子源性成分鉴定方法,同时通过双重PCR体系可一次反应同时扩增出狐狸和貉子条带,凝胶电泳条带整齐,背景清晰。结果表明:建立的狐狸和貉子动物源性PCR测定方法具有良好的特异性和灵敏度,其中狐狸引物单体系最低检测量为10 pg,貉子引物单体系最低检测量为1 pg,双重PCR体系由于引物的竞争作用使狐狸和貉子两种引物的检测灵敏度均降低至单体系的1/10。Two sets of specific primers were designed based on the conserved mitochondrial gene sequences of Alopex lagopus and Nyctereutes procyonoides and used to establish a PCR method to detect ingredients derived from A.lagopus and N.procyonoides in commercial meat products.Regular and clear A.lagopus-and N.procyonoides-specific bands were amplified simultaneously by duplex PCR.The sensitivities of the A.lagopus and N.procyonoides primers were respectively10pg and1pg in separate polymerase chain reactions,respectively.By contrast,the sensitivities of the A.lagopus and N.procyonoides primers were respectively100pg and10pg in duplex PCR due to the competition of two sets of primers.

关 键 词：狐狸 貉子 双重PCR 肉类掺假 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]