张氏马尾藻多糖的体外降胆固醇活性  

Cholesterol-lowering Activity of Sargassum zhangii Polysaccharide in vitro

作　　者：廖敏[1] 谌素华[1,2] 王维民[1] 钟思燕 俞素素 廖森泰[3] LIAO Min;WANG Wei-min;CHEN Su-hua;Zhong Si-yan;YU Su-su;LIAO Sen-tai(College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China;Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang 524088, China;Sericulture & Farm Produce Processing Research Institute, Guangdong Academy of Agricultural Sciences Guangzhou 510610, China)

机构地区：[1]广东海洋大学食品科技学院,广东湛江524088 [2]广东省水产品加工与安全重点实验室,广东湛江524088 [3]广东省农业科学院蚕业与农产品加工研究所,广东广州510610

出　　处：《广东海洋大学学报》2017年第3期80-85,共6页Journal of Guangdong Ocean University

基　　金：2015年广东省研究生教育创新计划项目(粤教研函[2016]1号);广东省自然科学基金项目(2015A030313618);广东海洋大学创新强校项目(GDOU2014041603);湛江市科技计划项目(2014A03017);2015年广东省联合培养研究生示范基地项目

摘　　要：采用超声波辅助水提法制得张氏马尾藻(Sargassum zhangii)粗多糖,用无水乙醇、丙酮、乙醚脱脂,Sevage法脱蛋白,得到张氏马尾藻初级纯化的多糖(ZSP),分析ZSP化学组成,模拟人体胃肠道pH环境,测定ZSP对胆固醇胶束中胆固醇的抑制作用及结合胆酸盐活性;采用MTT法观察ZSP对HepG2细胞生长影响,并研究ZSP浓度和作用时间对HepG2细胞内胆固醇水平影响。结果表明,在模拟人体胃肠道pH环境中,ZSP对胶束中胆固醇的抑制率高达18.74%,与牛磺胆酸钠、胆酸钠及甘氨胆酸钠的结合率分别高达57.69%、64.72%、69.03%;在48 h内,100、200、400、800、1 600μg/mL的ZSP对HepG2细胞的生长均无抑制作用;100、200、400、800μg/mL的ZSP在48 h内均可降低HepG2细胞内胆固醇水平,降低程度与外源性ZSP浓度和作用时间相关。ZSP具有良好的降胆固醇活性。Sargassum zhangii polysaccharide(ZSP)was extracted by ultrasonic wave extraction method,degreased by anhydrous ethanol,acetone and ether,and deproteinized by sevage method,then polysaccharide was purified preliminarily.ZSP chemical composition were analyzed,and the cholesterol-lowering activity of ZSP was examined.Simulating human gastrointestinal pH environment,the inhibition of cholesterol in the micelle cholestero with ZSP and the active of combination between cholate and ZSP were tested.The influence of ZSP on the growth of HepG2cells by MTT method was observed,and the impact of ZSP concentration and effective time on HepG2cells in cholesterol were studied.The results showed that in simulating pH environment,the inhibition rate of ZSP on cholesterol is as high as18.74%,and the rate of combination of ZSP with the taurocholic acid sodium,sodium cholic acid and sodium glycocholate is as high as57.69%,64.72%and57.69%respectively.Proliferation of HepG2cells could not be inhibited within48h when the ZSP concentration were100,200,400,800,and1600μg/mL,respectively,while ZSP with concentration of100,200,400,800μg/mL can lowered the cholesterol levels in the cells within48h,and the decrease was related to ZSP concentration and duration time.It is suggested that ZSP has a good cholesterol-lowering activity.

关 键 词：张氏马尾藻 多糖 降胆固醇活性 体外 胆酸盐 HEPG2细胞 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]