泡盛曲霉液体发酵产β-1,3-1,4-葡聚糖酶的条件优化  

Optimization of Liquid-State Fermentation Conditions for β-1,3-1,4-Glucanase (Lichenase) Production by Aspergillus awamori

作　　者：刘二伟[1] 杨绍青[2] 闫巧娟[3] 江正强[2] LIU Erwei;YANG Shaoqing;YAN Qiaojuan;JIANG Zhengqiang(College of Food Science and Bioengineering, Henan University of Science and Technology, Luoyang 471000, China;College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;College of Engineering, China Agricultural University, Beijing 100083, China)

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471000 [2]中国农业大学食品科学与营养工程学院,北京100083 [3]中国农业大学工学院,北京100083

出　　处：《食品科学》2017年第16期29-35,共7页Food Science

基　　金：国家自然科学基金面上项目(31471688)

摘　　要：从土壤样品中筛选得到一株高产β-1,3-1,4-葡聚糖酶的真菌,经鉴定为泡盛曲霉(Aspergillus awamori),命名为Aspergillus awamori CAU33。依次采用单因素试验和响应面分析法优化了其液体发酵产β-1,3-1,4-葡聚糖酶的条件,得到该菌株产酶的最适条件为:玉米芯质量浓度55 g/L、大豆蛋白胨质量浓度25 g/L、曲拉通X-114质量浓度23 g/L、初始pH 4.5、培养温度35℃、培养时间6 d。在此条件下β-1,3-1,4-葡聚糖酶活力达到8 447 U/m L,为优化前的17.6倍。The liquid-state fermentation conditions forβ-1,3-1,4-glucanase production by Aspergillus awamori CAU33,a fungal strain isolated from soil sample,were optimized using a combination of one-factor-at-a-time method and response surface methodology.The highestβ-1,3-1,4-glucanase activity of8447U/mL was achieved after6days of culture at35℃in a medium consisting of corncob55g/L,soybean peptone25g/L,and Triton X-11423g/L at an initial pH of4.5,about17.6times higher than before the optimization.

关 键 词：Β-1 3-1 4-葡聚糖酶 液体发酵 优化 泡盛曲霉 

分 类 号：TS201.3[轻工技术与工程—食品科学]