基于癸硫醇修饰的银纳米点阵列的苯并(a)芘检测  

Rapid Detection of Benzo (a) pyrene Using Decanethiol-Functionalized Ag Nanodot Arrays

作　　者：肖旺[1] 孙大文[1] 蒲洪彬[1] 韦庆益[1] XIAO Wang;SUN Dawen;PU Hongbin;WEI Qingyi(School of Food Science and Engineering, Academy of Contemporary Food Engineering,Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods,South China University of Technology, Guangzhou 510640, China)

机构地区：[1]华南理工大学食品科学与工程学院,现代食品工程研究中心,广东省冷链食品智能感知与过程控制工程技术研究中心,广东广州510640

出　　处：《食品科学》2017年第16期298-303,共6页Food Science

基　　金：广州市协同创新重大专项(201604020007)

摘　　要：利用阳极氧化铝模板法制备大面积高度有序的可调控的银纳米点阵列,并在其表面组装一层癸硫醇单分子层用于水中苯并(a)芘(benzo(a)pyrene,Ba P)的快速检测。首先,通过扫描电镜、紫外-可见透过光谱、罗丹明6G探针分子对不同扩孔时间制备的银纳米点阵列进行表征,结果表明扩孔时间为80 min制备的基底拉曼增强效果最好,增强因子可达1.6×10~6。然后,在此基底表面修饰一层癸硫醇分子,利用癸硫醇与BaP之间的疏水相互作用可以实现BaP的预富集,进而实现BaP的高灵敏度、稳定的检测。研究表明:利用此基底检测Ba P的检测限可达1.0 ng/mL,特征峰处的相对标准偏差为9.7%,满足高稳定性表面增强拉曼光谱基底的标准。该方法在BaP快速检测方面具有极大的潜力。A large-area,highly-ordered Ag nanodot array was prepared by anodic aluminum oxide(AAO)template method.A monolayer of decanethiol was then assembled on the surface of the nanodot array for the rapid detection of benzo(a)pyrene(BaP).The Ag nanodots array was characterized by scanning electron microscopy(SEM),UV-visible spectroscopy,and rhodamine6G probe.The results showed that the best Raman enhancement effect of surface enhanced Raman spectroscopy(SERS)-active substrate was achieved by80min pore broadening,with an enhancement factor of up to1.6×106.Then,a layer of decanethiol molecules was modified on the surface of the substrate.The pre-concentration of BaP could be achieved by the hydrophobic interaction between decanethiol and BaP.Sensitive and reproducible detection of BaP could be realized.The detection limit(LOD)of BaP was1.0ng/mL,and the relative standard deviation(RSD)of characteristic Raman peak was9.7%,which met the standard for a highly repeatable SERS substrate.This method has a great potential in the rapid detection of BaP.

关 键 词：表面增强拉曼光谱 苯并(A)芘 银纳米点阵列 阳极氧化铝 

分 类 号：O657.37[理学—分析化学]