居间驹形氏杆菌发酵大豆糖蜜生产细菌纤维素条件的优化  

Optimization of Bacterial Cellulose Production by Fermented Soybean Molasses with Komagataeibacter intermedius

作　　者：尹园[1,2] 马佳歌 倪春蕾 程建军[1] 徐速[1] YIN Yuan;MA Jiage;NI Chunlei;CHENG Jianjun;XU Su(College of Food Science, Northeast Agricultural University, Harbin 150030, China;Harbin Light Industry College, Harbin 150000, China)

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030 [2]哈尔滨轻工业学校,黑龙江哈尔滨150000

出　　处：《食品科学》2017年第18期8-16,共9页Food Science

基　　金："十二五"国家科技支撑计划项目(2014BAD22B01)

摘　　要：通过居间驹形氏杆菌(Komagataeibacter intermedius CGMCC12562)发酵生产细菌纤维素,以大豆糖蜜作主要发酵原料,优化发酵工艺参数,提高细菌纤维素产量。在单因素试验的基础上,筛选出对细菌纤维素产量影响较大的4个因素,即大豆糖蜜可溶性固形物含量、玉米蛋白粉、ZnSO_4及苹果酸添加量,并通过Design-Expert响应面分析对这4个因素进行优化,得到最优的培养基及发酵工艺为:大豆糖蜜可溶性固形物含量14.13°Brix、玉米蛋白粉添加量1.6%、ZnSO_4添加量0.11%、苹果酸添加量0.41%;在30℃、接种量10%、装料量16%、初始pH 6.0的条件下静置培养7d,细菌纤维素产量高达(15.68±0.82)g/L。In this study,Komagataeibacter intermedius CGMCC12562was employed to produce bacterial cellulose(BC)from soybean molasses.The fermentation process was optimized to improve BC yield.Through one-factor-at-a-time experiments,the soluble solids content(SSC)of soybean molasses,and corn gluten meal,ZnSO4and malic acid were selected as the factors with a significant influence on BC production for optimization by response surface methodology.It was found that the optimized culture medium consisted of soybean molasses with an SSC level of14.13°Brix,1.6%corn gluten meal,0.11%ZnSO4and0.41%malic acid.The BC yield was as high as(15.68±0.82)g/L after7days of culture at30℃for in a250-mL conical flask containing16%of the medium with an inoculum size of10%at an initial pH of6.0under static cultivation conditions.

关 键 词：大豆糖蜜 细菌纤维素 居间驹形氏杆菌 培养基 优化 

分 类 号：TS214.9[轻工技术与工程—粮食、油脂及植物蛋白工程]