不同饲养方式对苏尼特羊背最长肌肌纤维类型组成的影响  

Effect of Different Feeding Systems on Muscle Fiber Types of Longissimus dorsi from Sunit Sheep

作　　者：赵雅娟[1] 尹丽卿 苏琳[1] 刘树军 李云 王小斌 靳烨[1] ZHAO Yajuan;YIN Liqing;SU Lin;LIU Shujun;LI Yun;WANG Xiaobin;JIN Ye(College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China;Wulate Middle Banner Animal Husbandry Special-Purpose Facilitation Office, Wulate Middle Banner 015300, China)

机构地区：[1]内蒙古农业大学食品科学与工程学院,内蒙古呼和浩特010018 [2]乌拉特中旗农区畜牧业专项推进办公室,内蒙古乌拉特中旗015300

出　　处：《食品科学》2017年第19期30-34,共5页Food Science

基　　金：国家自然科学基金面上项目(31360393)

摘　　要：以12月龄不同饲养条件下(放牧和圈养)苏尼特羊背最长肌作为实验材料,用ATP酶染色法和实时定量聚合酶链式反应技术研究不同饲养方式下肌纤维类型组成。结果表明:放牧苏尼特羊背最长肌Ⅰ型和ⅡB型肌纤维直径和横截面积均显著小于圈养(P<0.05);同时,放牧苏尼特羊背最长肌Ⅰ型和ⅡA型肌纤维数量比例和面积比例、MyHCⅠ和MyHCⅡa基因的mRNA表达量均显著大于圈养(P<0.05),ⅡB型肌纤维数量比例和MyHCⅡb型基因mRNA表达量则呈现相反趋势。The aim of this study was to investigate the influence of different feeding systems(stall feeding and grazing)on muscle fiber types of longissimus doris from Sunit sheep by ATPase histochemical staining and real-time polymerase chain reaction.The results showed that the diameters and cross-sectional areas of typeⅠandⅡB muscle fibers in longissimus doris from grazing sheep were significantly lower than those from stall-fed sheep(P<0.05).The number and area percentages of typeⅠand typeⅡA,and MyHCⅠand MyHCⅡa gene expression in grazing sheep were significantly higher than those in stall-fed sheep(P<0.05),but the expression of typeⅡB and MyHCⅡb genes present the opposite trend.

关 键 词：苏尼特羊 饲养方式 肌纤维类型 MyHC基因 

分 类 号：TS251.5[轻工技术与工程—农产品加工及贮藏工程]