马尾藻岩藻聚糖硫酸酯组成分析及抗血栓活性评价  

Composition Analysis and Antithrombotic Activity of Fucoidan from Sargassum henslowianum C. Agardh

作　　者：廖敏[1] 王维民[1] 谌素华[1,2] 廖森泰 LIAO Min;WANG Weimin;CHEN Suhua;LIAO Sentai(College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China;Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang 524088, China;Sericulture & Farm Produce Processing Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China)

机构地区：[1]广东海洋大学食品科技学院,广东湛江524088 [2]广东省水产品加工与安全重点实验室,广东湛江524088 [3]广东省农业科学院蚕业与农产品加工研究所,广东广州510610

出　　处：《食品科学》2017年第19期189-194,共6页Food Science

基　　金：2015年广东省研究生教育创新计划项目(粤教研函[2016]1号);广东省自然科学基金项目(2015A030313618);广东海洋大学创新强校工程项目(GDOU2014041603);湛江市科技计划项目(2014A03017)

摘　　要：运用超声波热水浸提工艺从亨氏马尾藻中提取并经脱蛋白等初级纯化过程得到岩藻聚糖硫酸酯(F)。将F过DEAE C-52阴离子交换层析柱,得到次级纯化后的岩藻聚糖硫酸酯(F1)。对F、F1进行化学组成分析,用气相色谱-质谱联用方法测定其单糖组成。通过体外溶栓实验、小鼠肺栓塞实验以及出血实验对F及F1进行抗血栓活性评价。结果表明:与F相比,F1总糖含量、岩藻糖含量、硫酸基含量、单糖组成的种类均增多。中、高剂量的F、F1对血凝块有明显的溶解作用,且高剂量的血块溶解率显著高于尿激酶阳性对照组(P<0.05,P<0.01)。F及F1均能降低肺栓塞模型小鼠的死亡率,且高剂量的F1与模型对照组相比有极显著差异(P<0.01)。低、中、高剂量的F、F1均显著延长出血时间(P<0.05,P<0.01),F1低、中、高剂量组的出血时间均分别高于F各剂量组。F、F1各剂量组与肝素钠阳性对照组相比均有极显著差异(P<0.01),出血时间的延长率明显小于肝素钠对照组。说明马尾藻岩藻聚糖硫酸酯F、F1有较好的抗血栓活性,出血危险性较小,且F1抗血栓作用优于F。Fucoidan(F)from Sargassum henslowianum C.Agardh was extracted by ultrasonic-assisted hot water extraction and the extract was deproteinized by Sevag method and purified by DEAE C-52anion exchange column chromatography.The chemical compositions of the crude(F)and purified(F1)fucoidan were analyzed and their monosaccharide compositions were determined by gas chromatography-mass spectrometry(GC-MS).Meanwhile,antithrombotic activity was evaluated by thrombolysis test in vitro,pulmonary embolism test and hemorrhage test in mice.The results showed that the contents of total saccharide,fucose and SO42-in F1were increased and F1was composed of more monosaccharides when compared with F.The bleeding time of low,middle and high dose of F1were higher than each dose of F,and their effects at high dose were significantly higher than that of urokinase(P<0.05,P<0.01).Both F and F1could reduce the mortality of pulmonary embolism model mice,and there was a extremely significant difference between high-dose F1and the model group(P<0.01).F and F1could significantly prolong the bleeding time(P<0.05,P<0.01).Compared to the heparin sodium control group,all dose groups showed a extremely significant difference(P<0.01)and were significantly less effective in prolonging the bleeding time.These findings led to the conclusion that the crude and purified fucoidan from Sargassum henslowianum C.Agardh have good antithrombotic activity and cause little risk of bleeding and the antithrombotic effect of F1is better than that of F.

关 键 词：马尾藻 岩藻聚糖硫酸酯 抗血栓 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]