离子液体环境下淀粉的酶法疏水化改性  

Preparation of Hydrophobic Starch Through Lipase-Catalyzed Reaction in Ionic Liquids

作　　者：袁久刚[1,2] 向中林 范雪荣[3] 王强 姚金龙[1,2] 唐文君[1,2] 于拥军[1] YUAN Jiugang;XIANG Zhonglin;FAN Xuerong;WANG Qiang;YAO Jinlong;TANG Wenjun;YU Yongjun(Jiangsu Lianfa Textile Co .,L td ,Haian 226600,China;Key Laboratory o f Eco-Dyeing and Finishing,Jiangsu Province,Haian 226600,China;Schoo1 of Textiles and Clothing,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江苏联发纺织股份有限公司,江苏海安226600 [2]江苏省生态染整技术重点实验室,江苏海安226600 [3]江南大学纺织服装学院,江苏无锡214122

出　　处：《食品与生物技术学报》2017年第11期1152-1156,共5页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(31300785);江苏省企业博士创新计划项目(苏人才办[2014]27号);江苏省产学研-前瞻性联合研究项目(BY2014023-05)

摘　　要：本文作者采用脂肪酶Novozym 435在离子液体环境中对淀粉进行了月桂酸接枝改性,以提高其疏水性能。首先,对不同溶剂体系下的产物进行了取代度测试,并采用红外分析对产物的结构进行了表征,结果表明:在所选体系中,脂肪酶均能成功催化月桂酸与淀粉接枝,其中在离子液体中得到的产物取代度要高于其它体系。其次,对改性淀粉的性能进行了综合分析,改性后的淀粉其润湿接触角明显增大,疏水性提高,DSC测试结果表明改性淀粉耐热性能提高,糊化后淀粉的粘度和透光率都略有下降,SEM表明原淀粉的颗粒形态在改性过程中遭到了破坏,呈现出不规则的片状结构。In order to improve the hydrophobilicity of starch,lauric acid was grafted on starch through lipase-catalyzed reaction in ionic liquids.Firstly,the DS values of modified starch and their structure obtained in different solvent systems was detailed studied.Results showed that lauric acid could be successfully grafted on starch by Novozym435in all the selected systems.And therein,DS value of modified starch obtained in ionic liquids was much higher than the others.Secondly,the properties of modified starch were tested through water contact angle,DSC,viscosity and SEM.Results showed that the hydrophobicity of modified starch was significantly improved.DSC showed that melting temperature of modified starch was improved.In addition,viscosity and transmittance of the modified starch was slightly reduced accordingly.SEM showed that the surface morphology of starch particles was destroyed and became irregularly sheet structure during modification.

关 键 词：脂肪酶 淀粉 月桂酸 离子液体 

分 类 号：TS231[轻工技术与工程—粮食、油脂及植物蛋白工程]