黄芪对香菇菌丝体及子实体中成分的影响  

Effects of Astragalus on components of mycelia and fruiting body of Lentinus edodes

作　　者：张劲松[1] 牛宇 张丽珍[3] 单璐[3] 聂建军 徐全飞 冯婉君 潘保华 

机构地区：[1]山西大学生物技术研究所,山西太原030006 [2]山西省农业科学院农业资源与经济研究所,山西太原030006 [3]山西大学生命科学学院,山西太原030006

出　　处：《食品与发酵工业》2018年第2期113-120,共8页Food and Fermentation Industries

基　　金：白灵菇新品种选育及配套技术研究与应用"(FT2014-03-13);山西省农业科学院特色农业攻关项目"黄芪功能性食用菌的开发研究与栽培应用

摘　　要：研究了黄芪对香菇菌丝体和子实体中有效成分含量的影响,以紫外分光光度法测定总多糖、总酚酸、总黄酮、总皂苷含量,高效液相色谱法测定毛蕊异黄酮葡萄糖苷和芒柄花苷含量。结果表明,黄芪下脚料中的总多糖、总酚酸、总黄酮、总皂苷、毛蕊异黄酮葡萄糖苷和芒柄花苷含量分别为46.71 mg/g、2.62 mg/g、10.24 mg/g、10.03 mg/g、13.56μg/g、3.78μg/g,与黄芪药材相比成分倍数关系分别为3.91、1.89、1.77、2.05、5.21、5.24;黄芪水提物可以抑制香菇菌丝体的生长,随着质量浓度增大,菌丝生长抑制率升高,实验范围内不明显抑制香菇菌丝体生长的最大黄芪水提物质量浓度为0.5 g/100mL;在添加0.5 g/100mL黄芪水提物获得的香菌丝体中,胞内多糖、总酚酸、总黄酮、总皂苷的含量分别为52.52、0.97、0.35、11.11 mg/g,转化率分别为1.08%、2.20%、0.17%、13.56%;在袋栽时添加黄芪下脚料的香菇子实体中多糖、总酚酸、总黄酮、总皂苷的含量分别为60.48、1.16、0.68、3.56 mg/g,转化率分别为29.43%、6.26%、0.63%、9.09%;在添加黄芪水提物的菌丝体和添加黄芪下脚料子实体中均检测到毛蕊异黄酮葡萄糖苷,含量分别为1.09和0.86μg/g。The effects of Astragalus on the content of active ingredients of mycelia and fruiting bodies of Lentinus edodes were studied. The contents of total polysaccharides,total phenolic acids,total flavonoids and total saponins were determined by ultraviolet spectrophotometry. The content of calycosin-7-O-β-D-glucoside and fomononetin-7-Oglucoside were determined by high performance liquid chromatography. The results showed that the contents of total polysaccharides,total phenolic acids,total flavonoids,total saponins,calycosin-7-O-β-D-glucoside and fomononetin-7-O-glucoside in Astragalus waste were 46. 71 mg/g,2. 62 mg/g,10. 24 mg/g,10. 03 mg/g,13. 56 μg/g,and3. 78 μg/g,respetively. Compared with Astragalus,the multiple of the components was 3. 91,1. 89,1. 77,2. 05,5. 21,5. 24,respectively. The aqueous extract of Astragalus could inhibit the growth of mycelia of Lentinus edodes.Inhibition rate increased with the increasing concentration of aqueous extract of Astragalus. When the maximum concentration of aqueous extract of Astragalus membranaceus was 0. 5 g/100 m L,Lentinus edodes mycelium's growth was not significantly inhibited. In Lentinus edodes mycelium obtained from addition with 0. 5 g/100 m L of aqueous extract of Astragalus,the contents of total polysaccharides,total phenols,total saponins and total saponins were 52. 52 mg/g,0. 97 mg/g,0. 35 mg/g,11. 11 mg/g,the conversion rates were 1. 08 %,2. 20 %,0. 17 % and 13. 56 %,respectively. In Lentinus edodes fruiting bodies obtained after addition of Astragalus waste,the contents of polysaccharides,total phenolic acids,total flavonoids and total saponins were 60. 48 mg/g,1. 16 mg/g,0. 68 mg/g,3. 56 mg/g,the conversion rates were 29. 43 %,6. 26 %,0. 63 % and 9. 09 % respectively. In the experimental group,calycosin-7-O-β-D-glucoside were found in the mycelium and the fruiting bodies of Lentinus edodes with 1. 09 μg/g and 0. 86μg/g respectively.

关 键 词：黄芪 水提物 下脚料 香菇 深层发酵 菌丝体 子实体 

分 类 号：S646.12[农业科学—蔬菜学]