凤丹籽油对小鼠H22肿瘤的抑制作用  

Inhibitory Effect of Paeonia ostii Seed Oil on Hepatoma-22 Tumor Bearing Mice

作　　者：李梅青[1,2] 周鑫 王增蕾[1] LI Mei-qing;ZHOU Xin;WANG Zeng-lei(School of Tea&Food Science&Technology,Anhui Agricultural University,Hefei 230036,China;Hefei Institute of Agricultural Products Processin,Hefei 230036,China)

机构地区：[1]安徽农业大学茶与食品科技学院 [2]合肥农产品加工研究院,合肥230036

出　　处：《天然产物研究与开发》2018年第3期480-484,共5页Natural Product Research and Development

基　　金：安徽省高校自然基金重点项目(KJ2015A113)

摘　　要：探讨凤丹籽油对小鼠移植性肿瘤H22的抑瘤作用及机制。建立肝癌H22移植性肿瘤模型小鼠,随机分为正常组、模型组、5-氟尿嘧啶(5-FU)阳性对照组、凤丹籽油高、中、低剂量组,给药10 d后处死。结果表明:低、中、高剂量组的抑瘤率分别是18.4%、26.9%、34.7%,具有较好量效关系。与模型组相比,低、中、高剂量组能不同程度提高胸腺、脾脏指数,降低AST、ALT、ALP,增加WBC;降低TE活性和BCL-2表达,显著提高BAX表达。以上结果表明,高剂量凤丹籽油对H22荷瘤小鼠有明显的抑瘤作用,其机制可能是通过调节免疫功能和抑制TE活性、BCL-2表达,提高BAX表达而发挥肿瘤抑制作用。To explore the inhibitory effect of Paeonia ostii seed oil on transplanted H22 tumor in mice and its mechanism.H22 tumor bearing mouse models were established by transplanting H22 tumor into normal mice.48 mice were randomly divided into normal control,model,positive control,and low,medium,and high-dose P.ostii seed oil groups(at doses of 3.6,1.8,0.9 mL/kg,respectively)consisting of 8 mice each.Each mouse received intragastric administration for 10 consecutive days.The positive control group was administered intraperitoneally with 5-fluorouracil(5-FU)at doses of 20.0 mg/kg every day.After the last administration,all mice were sacrificed.The tumor growth inhibitory rate of low,medium and high-dose P.ostii seed oil groups were 18.4%,26.9%,34.7%respectively with a good dose-effect relationship.Compared with model group,the low,medium,and high-dose groups increased spleen and thymus indices at different degrees,decreased aspartate transaminase(AST),alanine aminotransferase(ALT),alkaline phosphatase(ALP)while improved white blood cell(WBC);suppressed the activity of telomerase(TE)and the expression of B-cell lymphoma-2(BCL-2)while raised the expression of Bcl-2 associated X protein(BAX)obviously.These results showed that high-dose P.ostii seed had significant anti-tumor effect on transplanted H22 tumor in mice and the possible mechanisms may be related to the regulation of immune function,inhibition of TE activity and up-regulating the expression of BAX and down-regulating the expression of BCL-2.

关 键 词：凤丹籽油 H22荷瘤小鼠 抑制作用 机制 

分 类 号：R730.52[医药卫生—肿瘤]