气相色谱法测定己酸菌发酵液中的己酸等有机酸含量  

Determination of Caproic Acid and Other Organic Acids in Fermented Liquid of Caproic Acid Bacteria by GC

作　　者：袁华伟[1,2,3] 杨泽刚 兰著玺 游玲 刘光钱[2] 胡永奇 王松涛[2,3] 沈才洪 YUAN Huawei;YANG Zegang;LAN Zhuxi;YOU Ling;LIU Guangqian;HU Yongqi;WANG Songtao;SHEN Caihong(College of Life Science and Food Engineering, Yibin University, Yibin, Sichuan 644000;Luzhou Laojiao Co. Ltd., Luzhou,Sichuan 646000;National Engineering Technology Research Center of Solid-state Brewing, Luzhou, Sichuan 646000, China)

机构地区：[1]宜宾学院生命科学与食品工程学院,四川宜宾644000 [2]泸州老窖股份有限公司,四川泸州646000 [3]国家固态酿造工程技术研究中心,四川泸州646000

出　　处：《酿酒科技》2018年第3期102-105,共4页Liquor-Making Science & Technology

基　　金：四川省科技计划项目<丢糟资源化利用技术开发与集成示范(重点)>(2017JY0110)

摘　　要：对气相色谱法测定己酸菌发酵液中己酸等有机酸含量的预处理方法进行了研究,优化了色谱条件,建立了一种快速测定己酸菌发酵液中己酸、丁酸、乙酸等有机酸的方法。己酸菌发酵液经2M硫酸酸化至p H2后经高速冷冻离心,再经微孔滤膜过滤,利用Pure-WAX毛细管色谱柱、以2-乙基丁酸为内标、直接进样气相色谱法定量测定。色谱分析条件为:检测器温度260℃,汽化温度250~℃,柱温150~℃,分流比50∶1,采样时间10 min。研究结果表明,应用该法测定己酸菌发酵液中的己酸、丁酸、乙酸等有机酸含量,RSD为2.22%~2.54%,加标回收率在96.83%~103.71%之间。该方法具有快速方便、定量准确等特点,适合己酸菌发酵液中己酸、丁酸、乙酸等有机酸的检测。The pretreatment for measuring the content of caproic acid and other organic acids in fermented liquid of caproic acid bacteria was studied.Firstly,the fermented liquid underwent2M sulfuric acid acidification till pH=2,then through high-speed freeze centrifugation and micropore filtration,direct sampling GC using Pure-WAX capillary-column and2-ethyl butyric acid as internal standard was performed.The chromatographic conditions were as follows:the detector temperature was at260℃,the vaporization temperature was at250℃,the column temperature was at150℃,the split ratio was50∶1and the sampling time was10min.The results showed that the content of caproic acid,butyric acid,acetic acid and other organic acids could be measured by such method,RSD was2.22%to2.54%,and standard addition recovery was96.83%to103.71%.This method had the advantages such as convenient,fast and accurate determination.

关 键 词：己酸菌发酵液 己酸 气相色谱法 检测 

分 类 号：TS262.3[轻工技术与工程—发酵工程] TS261.7[轻工技术与工程—食品科学与工程]