Effects of Nordy on the proliferation, differentiation,and apoptosis of HPV16 subgene-immortalized human endocervical cells  

作　　者：Wei Chen Yong Zhao Xiuwu Bian 

机构地区：[1]Department of Pathology, The Sixth People Hospital of Chongqing [2]Department of Pathology, Chongqing University of Medical Sciences [3]Institute of Pathology, Southwest Hospital, Army Medical University

出　　处：《Oncology and Translational Medicine》2018年第1期18-25,共8页肿瘤学与转化医学（英文版）

基　　金：Supported by a grant from the National 863 Project Guide Item(No.2002AA001010)

摘　　要：Objective The aim of this study was to investigate the effects of Nordy on the proliferation, differentiation, and apoptosis of HPV16 subgene-immortalized human endocervical cells(H8 cells).Methods After treatment with Nordy, H8 cell proliferation was evaluated using the MTT assay. The effects of Nordy on the cell cycle and apoptosis of H8 cells were analyzed by flow cytometry(FCM) and the Annexin V-FITC method. H8 cell MCM5 expression was detected by immunocytochemistry. Morphological changes were observed by light and electron microscopy. Telomerase activity was evaluated by TRAP-ELISA.Results We found that 10 μmol/L–100 μmol/L Nordy significantly inhibited H8 cell proliferation. After treatment with Nordy, H8 cells were blocked in the G0/G1 phase, and the rate of cell apoptosis increased significantly. Cells differentiated toward innocuousness, and MCM5 expression and telomerase activity notably decreased.Conclusion Nordy was observed to inhibit proliferation and promote apoptosis in H8 cells. Nordy also induced H8 cell differentiation; this effect may have been achieved by blocking the cell cycle and decreasing telomerase activity.Objective The aim of this study was to investigate the effects of Nordy on the proliferation, differentiation, and apoptosis of HPV16 subgene-immortalized human endocervical cells(H8 cells).Methods After treatment with Nordy, H8 cell proliferation was evaluated using the MTT assay. The effects of Nordy on the cell cycle and apoptosis of H8 cells were analyzed by flow cytometry(FCM) and the Annexin V-FITC method. H8 cell MCM5 expression was detected by immunocytochemistry. Morphological changes were observed by light and electron microscopy. Telomerase activity was evaluated by TRAP-ELISA.Results We found that 10 μmol/L–100 μmol/L Nordy significantly inhibited H8 cell proliferation. After treatment with Nordy, H8 cells were blocked in the G0/G1 phase, and the rate of cell apoptosis increased significantly. Cells differentiated toward innocuousness, and MCM5 expression and telomerase activity notably decreased.Conclusion Nordy was observed to inhibit proliferation and promote apoptosis in H8 cells. Nordy also induced H8 cell differentiation; this effect may have been achieved by blocking the cell cycle and decreasing telomerase activity.

关 键 词：induced DIFFERENTIATION CERVICAL cancer IMMORTALIZATION HPV 

分 类 号：R73[医药卫生—肿瘤]