糙米、米糠和精米在体外模拟消化过程中抗氧化活性的变化规律  

Changes in Antioxidant Activity Regularity in Brown Rice,Rice Bran and Polished Rice During In Vitro Simulated Digestion Process

作　　者：万红霞[1] 孙海燕[1] 赵旭 刘冬[1] 王慧清 WAN Hongxia;SUN Haiyan;ZHAO Xu;LIU Dong;WANG Huiqing(Shenzhen Polytechnic,Shenzhen 518055,China;Shenzhen Tengtai Rice Co.Ltd.,Shenzhen 518040,China)

机构地区：[1]深圳职业技术学院,广东深圳518055 [2]深圳市腾泰米业有限公司,广东深圳518040

出　　处：《食品与生物技术学报》2018年第1期93-99,共7页Journal of Food Science and Biotechnology

基　　金：广东省国际合作项目(2012B050600031);深圳市科技创新计划项目(CXZZ20130517145458596)

摘　　要：采用体外模拟消化法对糙米、米糠和精米进行模拟胃肠消化实验,采用Folin-ciocalteu法、氯化铝-亚硝酸钠法和氧自由基吸收能力法(ORAC)分别测定模拟消化过程中抗氧化活性物质(以多酚表示)、黄酮和抗氧化活性的变化规律。结果显示:与模拟胃消化0 h相比,模拟胃消化和模拟胃肠消化过程中糙米、米糠和精米的最大多酚释放量分别为其3.499、1.753和6.595倍和4.704、1.828、9.113倍,最大黄酮释放量分别为其的1.522、5.961和1.653倍和3.359、5.958、3.242倍,最大ORAC值分别为其3.459、4.849和12.93倍和5.659、5.112、19.65倍。表明谷物结合多酚在胃肠消化过程中,由于胃酸水解和胃肠蛋白酶酶解等消化作用使多酚由结合态转变为游离态,因而谷物结合多酚可以在胃肠道消化吸收。The simulated gastrointestinal digestive experiments were studied by the method of simulated digestion in vitro for brown rice,rice bran and polished rice,Regularity of antioxidant active substances(expressed in polyphenols)and flavonoids,as well as antioxidant activity were determined by Folin-ciocalteu method,aluminum chloride sodium nitrite and oxygen radical absorption capacity(ORAC)during in vitro simulated digestion process.The results showed that the maximum release of polyphenols for brown rice,rice bran and polished rice were 3.499-,1.753-,6.595-and 4.704-,1.828-,9.113-folder higher than that at the simulated gastric digestive time point 0 h during the simulated gastric digestion process and during simulated gastrointestinal digestion respectivly;the biggest release of flavonoids were 1.522,1.961,1.653 and 3.359,5.958,3.242 times more than that at 0 h as well as the maximum ORAC values raised 3.459,4.849,12.93 and 5.659,5.112,19.65 times respectivly.The results show that the grains bound polyphenols could transfer to free polyphenols during hydrochloric acid and enzymes hydolysis digestion and be available in the upper gastrointestinal tract.

关 键 词：稻米 体外模拟消化 多酚 黄酮 抗氧化活性 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]