黄肉桃果实八氢番茄红素脱氢酶基因的克隆及表达分析  

Cloning and Expression of Phytoene Desaturase Gene,PpPDS,from Peaches

作　　者：梁敏华 杨震峰[2] 苏新国[1] 宋春波 LIANG Min-hua;YANG Zhen-feng;SU Xin-guo;SONG Chun-bo(School of Food Science,Guangdong Food and Drug Vocational College,Guangzhou,Guangdong 510520,China;College of Biological and Environmental Sciences,Zhejiang Wanli university,Ningbo,Zhejiang 315100,China;College of Horticulture,South China Agricultural University,Guangzhou,Guangdong 510640,China)

机构地区：[1]广东食品药品职业学院食品学院,广东广州510520 [2]浙江万里学院生物与环境学院,浙江宁波315100 [3]华南农业大学园艺学院,广东广州510640

出　　处：《福建农业学报》2018年第6期608-613,共6页Fujian Journal of Agricultural Sciences

基　　金：国家自然科学基金(31371866);浙江省自然科学基金(LQ15C200004);广东省自然科学基金(2014A030313767);广州市科技计划项目(201804010417)

摘　　要：利用基因克隆技术获得桃果实八氢番茄红素脱氢酶基因(PDS)的全长核苷酸序列,命名为PpPDS。PpPDS全长2 056bp,编码区长度1 722bp,共编码573个氨基酸。进化树分析发现PpPDS与杏果实PaPDS、草莓FaPDS亲缘性较高。蛋白质序列分析表明PpPDS含有二核苷酸[NAD(H)/NADP(H)或FAD(H)]结合基元,其处于NAD(P)-binding Rossmann-like保守结构域内。实时荧光定量PCR结果显示,PpPDS基因在黄肉品种‘金丽’桃果实发育过程中均有表达,且在果肉中的表达普遍高于果皮。果实处于硬核期的PpPDS基因在果实中的表达显著高于软核果时期,随后其表达处于较高水平并呈平缓趋势。本研究对桃果实PpPDS基因的克隆及表达分析为研究桃果实类胡萝卜素生物合成分子机理奠定了良好的基础。The full-length cDNAs of phytoene desaturase(PDS)from peaches was isolatedby gene cloning,and named as PpPDS.It was 2 056 bp in length and encoded a predicted protein of 573 amino acids(ORF length at 1 722 bp).A phylogenetic analysis indicated that PpPDS closely resembled the PDS s from Prunus armeniaca and Fragaria×ananassa.The deduced amino acid sequence suggested that PpPDS protein contained dinucleotide binding motifs,NAD(H),NADP(H)or FAD(H),which belonged to the NAD(P)-binding rossmann-like conserved domain.Quantitative real-time PCR showed that PpPDS gene was generally expressed higher in mesocarp than exocarp in fruits of Jinli yellow peach at different maturity stages.After the peach stone hardened,the gene expression increased significantly,then leveled off at the peak.The information would be useful for further studies on the carotenoid biosynthesis pathway in peaches.

关 键 词：桃果实 PDS 基因克隆 表达分析 

分 类 号：S662.1[农业科学—果树学]