超高压协同温度处理对绿色魏斯氏菌的失活动力学  

Inactivation Kinetics of Weissella viridescens under Ultra-High Pressure Thermal Sterilization Treatment

作　　者：高芳[1,2,3] 李兴民[2] 马鹏飞 曾心[4] 徐宝才 GAO Fang;LI Xingmin;MA Pengfei;ZENG Xin;XU Baocai(National Institutes for Food and Drug Control,Beijing 102629,China;College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China;College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China;School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;State Key Laboratory of Meat Processing and Quality Control,Jiangsu Yurun Meat Group Co.Ltd.,Nanjing 210041,China)

机构地区：[1]中国食品药品检定研究院,北京102629 [2]中国农业大学食品科学与营养工程学院,北京100083 [3]南京农业大学食品科技学院,江苏南京210095 [4]江南大学食品学院,江苏无锡214122 [5]江苏雨润肉食品有限责任公司,肉品加工与质量控制国家重点实验室,江苏南京210041

出　　处：《食品科学》2018年第15期38-47,共10页Food Science

基　　金：国家自然科学基金面上项目(31571909)。

摘　　要：为了建立超高压协同温度处理对绿色魏斯氏菌在低温烟熏火腿中的失活模型,采用40、45、50℃结合200～500 MPa对接种10~8～10~9 CFU/mL绿色魏斯氏菌的烟熏火腿进行5、10、15、20、25、30 min的超高压处理,同时做相同条件的离体实验进行对比。采用薄层平板计数,以时间为横坐标,亡菌数量级为纵坐标作失活曲线,选择一级线性模型和非线性模型(Weibull和Logistic)进行模型的拟合,用模型的评价因子相关系数R^2、准确因子A_f和均方差验证方程的拟合度。结果表明:经热压结合处理后,接种于烟熏火腿中的绿色魏斯氏菌比相同条件下离体状态的绿色魏斯氏菌的失活更缓慢,可能因为火腿基质中存在一些大分子物质(如蛋白质、脂肪等)对菌体的保护作用。Logistic方程最适合描述绿色魏斯氏菌在两种状态下的失活情况,为预测绿色魏斯氏菌在烟熏火腿中的失活提供了理论依据。This work was undertaken to establish a model describing the inactivation of Weissella viridescens on lowtemperature smoked ham with ultra-high pressure thermal sterilization(HPTS).Weissella viridescens suspended in phosphate buffered saline and smoked ham inoculated with 108–109 CFU/mL of this strain were subjected to moderate heating(40,45 and 50℃)combined with high pressure(200–500 MPa)held for 5,10,15,20,25,30 min.The number of surviving cells was counted by the thin agar layer method.Inactivation curves describing the logarithmic value of the number of inactivated cells as a function of time were fitted with a first-order linear model and the Weibull and Logistic nonlinear models.The goodness of fit of the fitted models was examined by the correlation coefficient(R2),accuracy factor(Af)and mean square error(MSE).It was found that the inactivation of Weissella viridescens in situ was slower than in vitro after HPTS,which was due to the presence of macromolecular substances in the ham matrix including protein and fat,protecting bacteria from damage.Finally,the Logistic equation was the best model to describe bacterial inactivation in vitro and in situ,and could provide the theoretical foundation to predict Weissella viridescens inactivation on smoked hams.

关 键 词：超高压 绿色魏斯氏菌 烟熏火腿 离体 LOGISTIC模型 

分 类 号：TS251.6[轻工技术与工程—农产品加工及贮藏工程]