肉制品异源基因检测技术研究进展  

A Review of Recent Progress in Detection of Heterologous Genes in Meat Products

作　　者：胡谦 陈颖[2] 倪凯 葛兆方 曾海娟[1] 王淑娟[1] 马兰 刘箐[1] HU Qian;CHEN Ying;NI Kai;GE Zhaofang;ZENG Haijuan;WANG Shujuan;MA Lan;LIU Qing(School of Medical Instrument and Food Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China;Chinese Academy of Inspection and Quarantine,Beijing 100176,China;Xuzhou Green and Healthy Dairy Drinks Co.Ltd.,Xuzhou 221006,China)

机构地区：[1]上海理工大学医疗器械与食品学院,上海200093 [2]中国检验检疫科学研究院,北京100176 [3]徐州绿健乳品饮料有限公司,江苏徐州221006

出　　处：《食品科学》2018年第15期275-282,共8页Food Science

基　　金：国家自然科学基金面上项目(31371776);上海市科委"科技创新行动计划"长三角科技联合攻关领域项目(15395810900);徐州绿健乳品饮料有限公司"乳制品生产体系致病菌快速检测"项目(3A15308006)

摘　　要：近年来,食品掺假逐渐成为消费者关注的重要食品安全问题之一。由于利益的驱使,在肉制品行业异源肉质掺假现象尤其严重。目前,用于肉制品异源基因检测的技术包括普通聚合酶链式反应(polymerase chain reaction,PCR)技术、DNA指纹技术、实时荧光定量PCR技术、微滴式数字PCR技术、DNA条形码技术等。本文综述了肉制品中异源基因检测技术的研究进展,并对每种方法的优缺点和发展趋势予以讨论。In recent years,food adulteration has gradually become an important food safety issue of consumer concern.Driven by interests,adulteration with heterogeneous meat is a particularly serious problem in the meat processing industry.The current technologies available for the detection of heterologous genes in meat products include general polymerase chain reaction(PCR),DNA fingerprinting,real-time quantitative PCR,droplet digital PCR and DNA barcoding.This paper reviews recent progress in the development of technologies for the detection of heterologous genes in meat products,and discusses the advantages and disadvantages of each technology as well as future trend.

关 键 词：肉制品 异源基因 检测技术 

分 类 号：TS207.3[轻工技术与工程—食品科学]