基于酶技术生产低黏度及超低黏度褐藻胶的工艺  

Technology of producing low-viscosity and ultra-low-viscosity alginate by enzymatic method

作　　者：穆惠敏 沈照鹏[2,3] 林月 崔欣 孟蕾[4] 江晓路 MU Huimin;SHEN Zhaopeng;LIN Yue;CUI Xin;MENG Lei;JIANG Xiaolu(College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China;College of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China;Qingdao Marine Biomedical Research Institute,Qingdao 266071,China;State Key Laboratory of Bioactive Seaweed Substances,Qingdao Brightmoon Seaweed Group Co.Ltd,Qingdao 266400,China)

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003 [2]中国海洋大学医药学院,山东青岛266003 [3]青岛海洋生物医药研究院,山东青岛266071 [4]青岛明月海藻集团有限公司,海藻活性物质国家重点实验室,山东青岛266400

出　　处：《水产学报》2018年第9期1489-1496,共8页Journal of Fisheries of China

基　　金：“十三五”海洋经济创新发展示范产业链协同创新类项目

摘　　要：为解决当前低黏度及超低黏度褐藻胶生产工艺中存在的不足,本实验以海带为原料,利用褐藻胶裂解酶降解制备低黏度及超低黏度褐藻胶,研究了分子量、p H、温度对黏度的影响,确定了碱消化的最佳条件,探究了酶解工艺中加酶量、酶解时间及原料的初始黏度对褐藻胶产品的影响。结果显示,通过控制加酶量(100~500 U/g),酶解30 min即可得到低黏度及超低黏度褐藻胶,其中加酶量为100~330 U/g时可得到低黏度褐藻胶,加酶量增加至330~500 U/g时,可得到超低黏度褐藻胶,且酶解法得到的褐藻胶样品分子量均一度高,工艺节水率高达10%~50%;同时研究发现酶解样品黏度与原料初始黏度相关性不大,只在较短时间内表现出相关性,该工艺具有较高的原料适用性。In order to solve the problems in the alginate production process of low-viscosity and ultra-low-viscosity,in this study,we degraded Laminaria japonica(cultivated in Qingdao,Shandong)with align lyase to produce low-viscosity and ultra-low-viscosity aliginate and investigated the effects of molecular weight,pH and temperature on viscosity,then the optimum conditions for alkali digestion were determined.On this basis,we studied the effects of enzyme dosage,enzymolysis time and initial viscosity of raw materials on alginate products.The results showed that the low-viscosity and ultra-low-viscosity alginate can be obtained within 30 min.of enzymatic hydrolysis when the amount of enzyme was controlled within the range of 100~500 U/g,which the low-viscosity alginate can be obtained when the amount of enzyme was 100~330 U/g and the ultra-low-viscosity alginate can be obtained when the amount of enzyme was increased to 330~500 U/g.Meanwhile we found that the enzymatic hydrolysis of alginate samples was with high uniformity of molecular weight,and the water saving rate was as high as 10%~50%during the technology process.At the same time,it was found that the viscosity of the hydrolyzed sample was not correlated with the initial viscosity of the raw material,so the process had extensive applicability of the raw materials.

关 键 词：海带 酶解工艺 褐藻胶裂解酶 低黏度及超低黏度褐藻胶 

分 类 号：S68.421[农业科学—观赏园艺] Q14[农业科学—园艺学]