金针菇液态发酵培养初步研究  

Preliminary study on liquid fermentation of Flammulina velutipes

作　　者：丁霄霄 李凤伟 商曰玲 徐汉亿 王杰 卜雯丽 余晓红 DING Xiaoxiao;LI Fengwei;SHANG Yueling;XU Hanyi;WANG Jie;BU Wenli;YU Xiaohong(School of Marine and Bioengineering,Yancheng Institute of Technology,Yancheng 224051,China;School of Food and Biological Engineering,Jiangsu University,Zhenjiang 212013,China;Yancheng Yulu Agricultural Technology Co.,Ltd.,Yancheng 224000,China)

机构地区：[1]盐城工学院海洋与生物工程学院,江苏盐城224051 [2]江苏大学食品与生物工程学院,江苏镇江212013 [3]盐城市雨露农业科技有限公司,江苏盐城224000

出　　处：《中国酿造》2018年第9期124-129,共6页China Brewing

基　　金：江苏省科技厅苏北科技发展计划项目(富民强县)(BN2015123);江苏省六大人才高峰项目(No.2015-NY-022);江苏省社会发展项目(BE2018682);江苏省高校自然科学基金(18KJB550012)

摘　　要：以金针菇为试验材料,对液体种子发酵培养基的组分进行了筛选及优化。以pH值、还原糖、氨基酸态氮、酶活力、菌种生物量和菌丝球数目为评价指标,选择碳源、氮源、无机盐、维生素为影响因素,通过正交试验确定液体培养基配方。研究结果表明,金针菇的最佳液体培养基配方为:可溶性淀粉3%、黄豆粉5%、KH_2PO_4 0.05%、VB_2 0.005 0%。在此优化条件下,蛋白酶、纤维素酶和淀粉酶活力最高,分别为1.28 U/mL、1.25 U/mL和1.84 U/mL,相应的菌丝干质量为0.71 g/100 mL。Taking Flammulina velutipes as the tested material,the composition of liquid seed fermentation medium was screened and optimized.Using pH value,reducing sugar,amino acid nitrogen,enzyme activity,bacterial biomass and the number of mycelium pellet as evaluation indexes,and carbon source,nitrogen source,inorganic salt and vitamin as evaluation factors,the liquid medium formula was optimized by orthogonal tests.The results showed that the optimum formula of medium for F.velutipes was as follows:soluble starch 3%,soybean bean flour 5%,KH2PO4 0.05%,VB2 0.005 0%.Under these conditions,the protease activity,cellulase activity and amylase activity were the highest,which were 1.28 U/ml,1.25 U/ml and 1.84 U/ml,respectively.The corresponding dry weight of mycelium was 0.71 g/100 ml.

关 键 词：金针菇 液态发酵 培养基优化 生物量 

分 类 号：S646.15[农业科学—蔬菜学]