气相色谱法测定清香型白酒中乙酸乙酯和乙缩醛含量的研究  

Determination of ethyl acetate and acetal in Fen-flavor Baijiu by gas chromatography

作　　者：白宝清[1] 李颖星 金晓弟[1] 范三红[1] BAI Baoqing;LI Yingxin;JIN Xiaodi;FAN Sanhong(College of Life Sciences,Shanxi University,Taiyuan 030006,China)

机构地区：[1]山西大学生命科学学院,山西太原030006

出　　处：《中国酿造》2018年第9期181-184,共4页China Brewing

基　　金：山西省重点研发计划重点项目(201703D211019;201703D211012-1);山西省专利推广实施资助专项(20171002);2016年山西省高等学校教学改革创新项目(J2016003);2016年山西省研究生教育改革研究课题(2016JG26)

摘　　要：采用内标法气相色谱对清香型白酒中的乙酸乙酯和乙缩醛含量进行检测时,由于两者极性非常相似,在普通毛细管色谱柱上难以分离,因此通过对乙缩醛的酸性分解条件进行优化,实现白酒中乙酸乙酯和乙缩醛含量的同时测定。结果表明,当水浴温度30℃,水浴时间0.5 h,盐酸添加量为1%时,乙缩醛能充分分解。在此条件下,酒样中乙酸乙酯和乙缩醛在体积分数在0.01%~0.40%和0.005%~0.200%的范围内具有较好的线性关系(R^2>0.99),相对标准偏差(RSD)≤7.0%。6种清香型白酒样品中乙酸乙酯的含量为0.712~1.403 g/L,乙缩醛的含量为0.132~0.635 g/L。The content of ethyl acetate and acetal in Fen-flavor Baijiu(Chinese liquor)was detected with internal standard by gas chromatography.It was difficult to separate the ethyl acetate and acetal in the common capillary column due to very similar polarity.Therefore,the ethyl acetate and acetal in Baijiu samples can be simultaneous determined by optimizing the acidic decomposition conditions of acetal.Results showed that the optimal experimental conditions were water bath temperature 30℃,water bath time 0.5 h,and hydrochloric acid addition 1%,the content of ethyl acetate in the Baijiu sample was measured under the experimental condition.The results indicated that the calibration curves for the ethyl acetate and acetal displayed a good linearity(R2>0.99)in the volume concentration range of 0.01%-0.4%and 0.005%-0.2%,respectively.The relative standard deviations(RSD)were less than 7.0%.The ethyl acetate contents in 6 kinds of Fen-flavor Baijiu were 0.712-1.403 g/L,and the acetal contents were 0.132-0.635 g/L.

关 键 词：气相色谱 乙酸乙酯 乙缩醛 清香型白酒 内标法 

分 类 号：TS261.7[轻工技术与工程—发酵工程]