不同微生物对魔芋低聚甘露糖的降解与利用比较  

Investigation on the regular patterns of konjac mannan oligosaccharide utilization by different bacteria

作　　者：蒋敏 王苗 李恒 史劲松 JIANG Min;WANG Miao;LI Heng;SHI Jin-song(School of Pharmaceutical Science,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学药学院,江苏无锡214122

出　　处：《食品与发酵工业》2018年第10期14-19,共6页Food and Fermentation Industries

基　　金："十二五"国家科技支撑项目(2012BAD33B06)功能低聚糖的规模化制备关键技术及其产业示范

摘　　要：以体外静态培养的方式探讨了3株不同属微生物(青春双歧杆菌Bifidobacterium adolescentis、单形拟杆菌Bacteroides uniformis、约氏乳杆菌Lactobacillus johnsonii)对魔芋低聚甘露糖(konjac mannon oligosaccharide,KMOS)的降解利用规律及代谢产物产生情况。结果表明,Bacteroides uniformis能快速且完全利用DP 2～3组分,Lactobacillus johnsonii虽然也能完全利用这2个组分,但是利用效率较慢且具有明显的迟滞效应;而Bifidobacterium adolescentis则只能完全利用DP 2组分,对DP 3～4组分的利用不完全且有一定迟滞性。3株菌在含KMOS的发酵培养基中均能积累有机酸和短链脂肪酸(short chain fatty acid,SCFA),但不同菌的产酸规律存在一定差异。The regular patterns of KMOS utilization by three different bacteria(Bifidobacterium adolescentis,Bacteroides uniformi,Lactobacillus johnsonii)was investigated under anaerobic and static conditions.The results showed that Bacteroides uniformis could quickly and completely consumed DP 2~3,whereas Lactobacillus johnsonii could utilize these components slowly but with a significant lag phenomenon.Different with the former two,Bifidobacterium adolescentis could only consume DP 2 completely and DP 3~4 partly.All of the 3 bacteria could grow in the culture with KMOS and produce organic acid(mainly lactic acid and succinic acid)and SCFA(mainly acetic acid,butanoic acid,butylcarboxylic acid and hexylic acid),but had significant discrepancy in the rate and content.These results could provide reference for developing prebiotics product of KMOS.

关 键 词：青春双歧杆菌 单形拟杆菌 约氏乳杆菌 魔芋低聚甘露糖 短链脂肪酸 有机酸 

分 类 号：TS201.3[轻工技术与工程—食品科学]