部分脱脂亚麻籽粉对高脂高糖饮食大鼠血糖的影响  

Effect of Partially Defatted Flaxseed Meal on Blood Glucose in Rats Fed with High-Fat High-Sugar Diet

作　　者：李培培 黄庆德[1] 许继取[1] 张亚丽[1] 马丛丛[1] 邓乾春[1] LI Peipei;HUANG Qingde;XU Jiqu;ZHANG Yali;MA Congcong;DENG Qianchun(Oil Crops and Lipids Process Technology National&Local Joint Engineering Laboratory,Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,China)

机构地区：[1]中国农业科学院油料作物研究所,油料油脂加工技术国家地方联合工程实验室,湖北武汉430062

出　　处：《食品科学》2018年第21期183-188,共6页Food Science

基　　金：现代农业产业技术体系建设专项(CARS-14)。

摘　　要：亚麻籽压榨制油后的亚麻饼深度开发利用研究受到关注。本实验将亚麻饼加工成部分脱脂亚麻籽粉(partially defatted flaxseed meal,PDFM),研究PDFM干预对高脂高糖饲料大鼠血糖的影响。将SD大鼠分成6组(n=9):正常对照组、高糖高脂模型组、阿卡波糖阳性治疗对照组和PDFM低(质量分数5%PDFM)、中(质量分数10%PDFM)、高(质量分数20%PDFM)剂量组。8周后对大鼠空腹血糖值进行测定,并进行糖耐量实验,然后处死大鼠并解剖,观察内脏,测定肝脏指数、胰指数和腹部脂肪指数,取血检测糖化血红蛋白、肝糖原和血脂浓度。结果表明:1)动物空腹血糖值,高脂高糖模型组和低、中、高剂量PDFM组分别为(7.15±1.22)、(6.34±0.48)、(5.82±0.36)、(5.03±0.32)mmol/L,呈依次下降趋势,超过7.0 mmol/L的糖尿病判定标准大鼠比例分别为5/9、1/9、0、0,表明PDFM可以抑制高脂高糖饮食造成的动物血糖升高和糖尿病形成,在抑制动物血糖升高方面呈明显的剂量效应关系,PDFM剂量越高对血糖升高的抑制作用越强,其中,中、高剂量PDFM组抑制血糖升高效果与高脂高糖模型组对照达到显著水平(P<0.05);2)与阿卡波糖阳性治疗对照组空腹血糖值((5.14±0.33)mmol/L)相比,中、高剂量PDFM组达到阿卡波糖抑制血糖升高效果。综合考虑PDFM对糖化血红蛋白、肝糖原、肝脏指数、血脂等影响结果,PDFM具有抑制血糖升高功能活性,具备开发成为辅助降血糖制品原料的潜力。Currently,there is considerable interest in the utilization of flaxseed cake.The effect of partially defatted flaxseed meal(PDFM)processed from flaxseed cake on blood sugar levels in rats fed with a high-fat high-sugar diet was investigated in the present study.SD rats were divided into 6 groups(n=9):normal,high-fat high-sugar model,acarbose treatment(positive control),low-dose PDFM(5%PDFM),middle-dose PDFM(10%PDFM)and high-dose PDFM(20%PDFM)groups.After intervention for 8 weeks,fasting blood glucose was measured,and glucose tolerance test was carried out.Then,the rats were sacrificed and dissected to observe the internal organs and determine liver index,pancreas index and abdominal fat index as well as glycosylated hemoglobin,liver glycogen and serum lipid.The results showed that 1)fasting blood glucose levels in the model,low-,medium-,and high-dose PDFM groups were(7.15±1.22),(6.34±0.48),(5.82±0.36),and(5.03±0.32)mmol/L,respectively,which were found to contain 5,1,0 and 0 rats with fasting blood glucose levels greater than 7.0 mmol/L,indicating that PDFM could inhibit hyperglycemia and diabetes development in high-fat highsugar diet fed rats,and the anti-hyperglycemia effect was highly dose dependent and significant in the middle-and high-dose groups compared with the model group(P<0.05);2)middle-and high-dose PDFM was as effective as acarbose in reducing reduced fasting blood glucose,which was(5.14±0.33)mmol/L in the acarbose treatment group.Taking into consideration its effect on all above parameters,PDFM has great potential as an auxiliary hypoglycemic agent.

关 键 词：亚麻饼 部分脱脂亚麻籽粉 动物实验 血糖 糖化血红蛋白 

分 类 号：S565.9[农业科学—作物学]