基于竞争性等位基因特异性PCR技术的麦芽品种纯度定性和定量检测  

Kompetitive Allele Specific PCR(KASP) for the Qualification and Quantification of Malt Varieties

作　　者：蒋培基 王德良[2] 徐东东[3] 黄承雪 郭刚刚[3] 栾春光[2] 蒲彪[1] JIANG Peiji;WANG Deliang;XU Dongdong;HUANG Chengxue;GUO Ganggang;LUAN Chunguang;PU Biao(College of Food Science, Sichuan Agricultural University, Ya’an 625014, China;China National Research Institute of Food and Fermentation Industries, Beijing 100015, China;Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

机构地区：[1]四川农业大学食品学院,四川雅安625014 [2]中国食品发酵工业研究院,北京100015 [3]中国农业科学院作物科学研究所,北京100081

出　　处：《食品科学》2018年第24期322-326,共5页Food Science

摘　　要：利用测序技术筛选和确认4个可以区分7种大麦麦芽的单核苷酸多态性(single nucleotide polymorphisms,SNP)位点,并建立区分图谱。同时,利用竞争性等位基因特异性聚合酶链式反应检测技术对预混样品进行定量测试,其检测的结果与真实性之间相对误差小于3%。该技术的建立对大麦麦芽纯度检测提供方法,对大麦麦芽SNP指纹数据库的完善提供了数据支持。In this study,4single nucleotide polymorphisms(SNPs)were screened out and identified by sequencing for the discrimination of7malt varieties.Furthermore,discrimination profiles were also established using SNP based kompetitive allele specific PCR(KASP)assay.Quantitative analysis of known mixed samples was carried out using the KASP assay with a relative error less than3%between the measured and actual values.The KASP assay is useful for the enrichment of malt SNP fingerprint database.

关 键 词：大麦麦芽 竞争性等位基因特异性PCR(KASP) 定性检测 定量检测 

分 类 号：TS261[轻工技术与工程—发酵工程]