超高效液相色谱-静电场轨道阱高分辨质谱法测定茶叶中21种农药残留量  

Determination of 21 Pesticide Residues in Tea by Ultra Performance Liquid Chromatography Orbitrap High Resolution Mass Spectrometry

作　　者：金莉莉[1,2] 郝振霞[1] 高贯威 柴云峰[1] 王晨[1] 陈红平[1] 鲁成银[1] JIN Lili;HAO Zhenxia;GAO Guanwei;CHAI Yunfeng;WANG Chen;CHEN Hongping;LU Chengyin(Tea Research Institute,Chinese Academy of Agricultural Sciences,Laboratory of Quality and Safety Risk Assessment for Tea Pr oducts(Hangzhou),Key Laboratory of Tea Quality and Safety Control,Ministry of Agri culture,Hangzhou 310008,China;Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]中国农业科学院茶叶研究所,农业部茶叶产品质量安全风险评估实验室(杭州),农业部茶叶质量安全控制重点实验室,浙江杭州310008 [2]中国农业科学院研究生院,北京100081

出　　处：《茶叶科学》2018年第6期595-605,共11页Journal of Tea Science

基　　金：中国农业科学院创新团队茶叶质量与风险评估团队(CAAS-ASTIP-2014-TRICAAS-06);国家自然科学基金项目(31601554);浙江省自然科学基金项目(LQ16C200006);浙江省公益应用项目(2017C32059);现代农业产业技术体系建设专项基金资助项目(nycytx-26)。

摘　　要：采用超高效液相色谱-静电场轨道阱高分辨质谱,建立了茶叶中21种农药残留量的检测方法。茶叶样品经甲醇和水(V甲醇∶V水=1∶1)提取后,采用乙二胺-N-丙基硅烷和强阳离子交换剂作为混合吸附剂进行萃取净化。以C18色谱柱进行色谱分离,采用FullScan扫描模式进行定性、定量分析。结果显示,21种农药在0.5～200μg·L^(-1)范围内均呈良好的线性关系,相关系数(r2)大于0.99。在10、50、100μg·kg^(-1)3个加标水平下,平均回收率为70%～125%之间,相对标准偏差(RSD)小于15%,定量限为10μg·kg^(-1)。本方法操作简单快速,灵敏度高,适用于茶叶中21种农药残留检测。A ultra performance liquid chromatography orbitrap high resolution mass spectrometry method for the determination of 21 pesticides in tea was developed.The tea samples were extracted by methanol–water(Vmethanol∶Vwater=1∶1),and cleaned up by a mixture of adsorbents containing primary secondary amine and strong cation exchanger.The chromatography analysis was performed on a C18 column.The target analytes were analyzed by high resolution mass spectrometry under Full Scan mode.The results showed that the linearities of 21 pesticides ranged from 0.5μg·L^-1 to 200μg·L^-1 with correlation coefficients higher than 0.99.At the spiked levels of 10,50 and 100μg·kg^-1,the average recoveries were from 70%to 125%,with the relative standard deviations(RSDs)below 15%.The limits of quantitation were 10μg·kg^-1.The method is simple,rapid,sensitive and appropriate for the determination of 21 pesticide residues in tea.

关 键 词：茶叶 农药残留 超高效液相色谱-静电场轨道阱高分辨质谱 

分 类 号：TS272[农业科学—茶叶生产加工] O657.7[轻工技术与工程—农产品加工及贮藏工程]