中链甘油三酯/玉米醇溶蛋白核壳结构微胶囊载体的构建及表征  

Construction and Characterization of Medium-Chain Triglyceride(MCT)/Zein Microcapsules with Core-Shell Structure

作　　者：黄雪 王颖[1] 张慧 赵萌[1,2] 方亚鹏 HUANG Xue;WANG Ying;ZHANG Hui;ZHAO Meng;FANG Yapeng(Glyn O.Phillips Hydrocolloid Research Centre,Hubei International Scientific and Technological Cooperation Base of Food Hydrocolloids,Hubei University of Technology,Wuhan 430068,China;Hubei Collaborative Innovation Centre for Industrial Fermentation,Wuhan 430068,China)

机构地区：[1]湖北工业大学菲利普斯亲水胶体研究中心湖北省食品胶体国际科技合作基地,湖北武汉430068 [2]工业发酵湖北省协同创新中心,湖北武汉430068

出　　处：《食品科学》2019年第4期21-27,共7页Food Science

基　　金：国家自然科学基金青年科学基金项目(31601514);国家自然科学基金面上项目(31322043);湖北省科技厅国际合作项目(DWHZ2017000010)

摘　　要：以中链甘油三酯(medium-chain triglyceride,MCT)为油相内核,以玉米醇溶蛋白(Zein)为外壳主要成分,低剪切条件下反溶剂法制备大粒径核壳结构的MCT/Zein微胶囊微米载体。通过考察微胶囊载体的宏观形态、微观形态及粒径分布,优化得到微胶囊载体的最佳制备工艺为质量分数0.5%的阿拉伯胶(gum arabic,GA)作为乳化剂、乳化速率800 r/min、Zein沉淀溶液为"30 mL水+100 mL GA溶液"、Zein沉淀溶液加入速率6 mL/min,并采用扫描电子显微镜、激光共聚焦显微镜、激光粒度分析仪表征冻干MCT/Zein微胶囊载体的结构特征和粒径特性,结果表明该法制备的微胶囊载体具有典型的核壳结构,粒径均一、稳定性良好。本实验建立MCT/Zein核壳结构微胶囊的反溶剂制备法,该法简单易行,适用于多种油溶性活性组分的包埋和保护研究。In this research,medium-chain triglyceride(MCT)and zein were used respectively as internal oil phase(core)and external shell to prepare MCT/zein microcapsules with core-shell structure by the anti-solvent method.Based on the macro-morphology,micro-morphology and size distribution of microcapsules,the optimum preparation conditions were determined as follows:0.5%gum arabic(GA)as emulsifier,emulsification speed 800 r/min,zein precipitation with a mixture of 30 mL of water and 100 mL of GA solution added at a rate of 6 mL/min.Scanning electron microscopy,confocal laser scanning microscopy and laser particle size analyzer were used to characterize the structure and size properties of freeze-dried MCT/zein microcapsules.It turned out that the MCT/zein microcapsules had a typical core-shell structure with homogenous size distribution and good storage stability.In conclusion,the anti-solvent method was simple,practical and suitable for the preparation of MCT/zein microcapsules with core-shell structure for application in the encapsulation and protection of various lipophilic bioactive components.

关 键 词：微胶囊载体 核壳结构 反溶剂法 中链甘油三酯 玉米醇溶蛋白 

分 类 号：TS201.1[轻工技术与工程—食品科学]