大肠杆菌利用葡萄糖和木糖合成乙醇酸、乳酸和3-羟基丁酸共聚酯  

Microbial production of poly(glycolate-co-lactate-co-3-hydroxybutyrate) from glucose and xylose by Escherichia coli

作　　者：笪央央 李微 史理陇 李正军[1] Yangyang Da;Wei Li;Lilong Shi;Zhengjun Li(College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China)

机构地区：[1]北京化工大学生命科学与技术学院,北京100029

出　　处：《生物工程学报》2019年第2期254-262,共9页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No.21476014);国家科技支撑计划(No.2015BAD15B09)资助~~

摘　　要：以大肠杆菌为宿主,构建了以葡萄糖和木糖为底物获得乙醇酸、乳酸和3-羟基丁酸共聚酯的生物合成途径,包括过表达塔格糖-3-差向异构酶、核酮糖激酶、醛缩酶、醛脱氢酶、丙酰辅酶A转移酶、β-酮硫解酶、乙酰乙酰辅酶A还原酶和聚合酶等。在此基础上,表达聚羟基脂肪酸酯颗粒结合蛋白,提高了聚合物的合成,重组菌的细胞干重达到3.73g/L,含有38.72wt%的共聚酯。采用混菌共培养策略,实现以葡萄糖和木糖混合物为底物合成共聚酯,摇瓶实验中细胞干重达到4.01g/L,含有21.54wt%的聚合物。文中提供了一种以葡萄糖和木糖混合物为碳源合成聚合物的方法,为下一步纤维素水解物的有效利用提供了参考。Escherichia coli was metabolically engineered to produce poly(glycolate-co-lactate-co-3-hydroxybutyrate) using glucose and xylose as carbon sources. The combinatorial biosynthetic route was constructed by the overexpression of a series of enzymes including D-tagatose 3-epimerase, L-fuculokinase, L-fuculose-phosphate aldolase, aldehyde dehydrogenase, propionyl-CoA transferase,β-ketothiolase, acetoacetyl-CoA reductase, and polyhydroxyalkanoate synthase. Overexpression of polyhydroxyalkanoate granule associated protein significantly improved biopolymer synthesis, and the recombinant strain reached 3.73 g/L cell dry weight with 38.72%(W/W) biopolymer content. A co-culture engineering strategy was developed to produce biopolymer from a mixture of glucose and xylose, achieving 4.01 g/L cell dry weight containing 21.54%(W/W) biopolymer. The results of this work offer an approach for simultaneously utilizing glucose and xylose and indicate the potential for future biopolymer production from lignocellulosic biomass.

关 键 词：聚羟基脂肪酸酯 乙醇酸 乳酸 3-羟基丁酸 葡萄糖 木糖 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]