响应面法优化双孢菇鲜味物质提取工艺的研究  

Study on Optimization of Extraction Process of Aroma Flavor Substance from Agaricus bisporus by Response Surface Method

作　　者：卢朝婷 刘江 王晓君 沈秋霞 刘洪 李明元 吉礼 LU Chao-ting;LIU Jiang;WANG Xiao-jun;SHEN Qiu-xia;LIU Hong;LI Ming-yuan;JI Li(School of Food and Bioengineering,Xihua University,Chengdu 610039,Sichuan,China;Sichuan Food Production Safety Association,Chengdu 610061,Sichuan,China)

机构地区：[1]西华大学食品与生物工程学院,四川成都610039 [2]四川省食品生产安全协会,四川成都610061

出　　处：《食品研究与开发》2019年第6期88-94,共7页Food Research and Development

基　　金：四川省科技计划(2017KFP0216)

摘　　要：以α-氨基氮含量和蛋白质含量为指标,在考察加酶量、酶解时间、酶解温度和酶解pH值4个单因素对双孢菇风味料提取率影响的基础上,采用Box-Behnken响应面设计法,选取酶添加量、酶解温度和酶解时间为自变量,通过采取中心组合设计的方法,研究各自变量交互作用及对双孢菇风味料提取率的影响。结果表明:纤维素酶解法提取双孢菇香味物质的最佳工艺条件为:纤维素酶加酶量1 257.04 U/g、酶解时间110.09 min、酶解温度50.2℃。结合实际,调整各因素为:加酶量1 000 U/g、酶解时间110 min、酶解温度50℃。此条件下进行3次平行试验验证,α-氨基氮平均含量为27.94 mg/g,与预测值基本相符,证明该模型可靠。α-Amino nitrogen content and protein content were used as indicators based on the effection of four single factors including the different enzyme dosage,enzymolysis time,enzymolysis temperature and enzymolysis pH value on the extraction rate of Agaricus bisporus flavourings.Box-behnken response surface design method was adopted to select the amount of enzyme,the temperature of enzymolysis and the time of enzymolysis as independent variables.The interaction of each variable and their effects on the extraction rate of Agaricus bisporus was studied by central combination deign.The results showed that the cellulose enzymatic hydrolysis extraction of Agaricus bisporus optimum process conditions of flavoring substances of cellulase enzyme dosage 1 257.04 U/g,enzymolysis time 110.09 min,enzyme solution temperature 50.2℃.Combined with the actual conditions,adjusting the factors as follows:enzyme dosage 1 000 U/g,enzymolysis time 110 min,enzymolysis temperature 50℃.In this condition,theα-amino nitrogen average content of these three parallel experiments was 27.94 mg/g,which basically consistented with the predicted value,proving the model was reliable.

关 键 词：双孢菇 纤维素酶 响应面 Α-氨基氮 工艺优化 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]