铜绿假单胞菌产抗菌代谢产物发酵条件的优化  

Optimization of Fermentation Conditions for Antibacterial Metabolites Produced by Pseudomonas aeruginosa

作　　者：韩志雪 程泓森 倪孟祥[1] HAN Zhixue;CHENG Hongsen;NI Mengxiang(College of Life Science and Technology,China Pharmaceutical University,Nanjing 210000,China;Nanjing First High School,Nanjing 210000,China)

机构地区：[1]中国药科大学生命科学与技术学院,江苏南京210000 [2]南京市第一中学,江苏南京210000

出　　处：《化学与生物工程》2019年第3期41-46,共6页Chemistry & Bioengineering

摘　　要：以分离的铜绿假单胞菌作为实验菌株,以对耐甲氧西林金黄色葡萄球菌(MRSA)的抑制效果及菌体干重为评价指标,采用单因素实验、正交实验优化铜绿假单胞菌产抗菌物质的培养基组分和发酵条件。结果表明:优化的培养基组分为黄豆粉2.5 g·L^(-1)、大豆蛋白胨10 g·L^(-1)、磷酸氢二钠10 g·L^(-1),优化的发酵条件为接种量2%、初始pH值7、发酵时间96 h。Using the isolated Pseudomonas aeruginosa as an experimental strain,the inhibition effect of the strain on Methicillin-resistant Staphylococcus aureus (MASR)and the dry weight of the bacteria as evaluation indexes,we optimized medium components and fermentation conditions by the single-factor experiment and orthogonal experiment.The results show that the optimal medium components are as follows:soybean powder of 2.5 g·L^-1,soybean peptone of 10 g·L^-1,and disodium hydrogen phosphate of 10 g·L^-1;the optimal fermentation conditions are as follows:inoculum of 2%,initial pH value of 7,and fermentation time of 96 h.

关 键 词：铜绿假单胞菌 培养基组分 发酵条件 抑菌活性 

分 类 号：TQ920.6[轻工技术与工程—发酵工程] TQ423