响应面法优化微波辅助提取蛤蟆草蛋白工艺研究  

Optimization of Microwave-Assisted Extraction Process of Protein from Bothriospermum chinense by Response Surface Methodology

作　　者：李西腾[1] 皮林林 张佳佳[1] 李帅 曹献鑫 LI Xiteng;PI Linlin;ZHANG Jiajia;LI Shuai;CAO Xianxin(School of Food, Jia ngsu Food & Phannaceutical Science College (Huai'an 223003))

机构地区：[1]江苏食品药品职业技术学院食品学院,淮安223003

出　　处：《食品工业》2019年第1期52-55,共4页The Food Industry

基　　金：江苏高校品牌专业建设工程资助项目(PPZY2015B19)

摘　　要：以蛤蟆草为原料,以蛋白提取量为指标,采用微波辅助提取蛤蟆草中蛋白。分别研究了液料比、微波功率、微波提取时间、乙醇体积分数对蛋白提取量的影响,通过响应面试验优化了提取工艺。结果表明,蛤蟆草蛋白提取的较佳工艺条件是:液料比50︰1 mL/g,微波功率460 W,微波提取时间3 min,乙醇体积分数60%。在该条件下,蛤蟆草蛋白的提取量为1.86 mg/g。With Bothriospermum chinense as raw material, extraction content of protein as the index, Bothriospermum chinenseprotein was extracted through microwave-assisted method. The effects of liquid-solid ratio, microwave power, microwave extraction time, and ethanol concentration on the extraction of protein were studied. The results showed that the optimum extraction conditions by response surface method were: liquid-solid ratio 50︰1 mL/g, microwave power 460 W, microwave extraction time 3 min, and ethanol concentration 60%. Under these conditions, the extraction of Bothriospermum chinense protein was 1.86 mg/g.

关 键 词：蛤蟆草 蛋白提取 微波辅助 响应面法 

分 类 号：R284.2[医药卫生—中药学]