超声波法提取甜叶菊中甜菊糖苷的最佳工艺条件研究  

Study on the Optimum Extraction Process of Steviol Glycosides from Stevia rebaudiana Leaves by Ultrasonic Method

作　　者：钟利叶 牟志珍 杨传辉[1] 王文君[1] 邓镇涛[1] ZHONG Liye;MOU Zhizhen;YANG Chuanhui;WANG Wenjun;DENG Zhentao(Bioengineering Faculty, Zuiyi Medical University Zhuhai Campus (Zhuhai 519041))

机构地区：[1]遵义医学院珠海校区生物工程系,珠海519041

出　　处：《食品工业》2019年第1期82-86,共5页The Food Industry

基　　金：地方高校国家级大学生创新创业训练计划项目(201710661047);贵州省级大学生创新创业训练计划项目(201710661047);校级大学生创新创业训练计划项目(ZHCX20160005)

摘　　要：试验以甜叶菊干叶为原料,在单因素试验基础上选择影响显著的因素进行正交试验,研究甜菊糖苷的超声波法最佳提取工艺条件,用蒽酮比色法进行定量分析。结果表明:最佳提取工艺条件为料液比1︰60 (g/mL)、超声时间60 min、超声功率150 W、超声温度55℃、提取次数3次,甜菊糖苷提取率为27.32%。For studing on the optimum conditions for the extraction of steviol glycosides from Stevia rebaudiana leaves by ultrasonic method, the orthogonal experiment was done on the basis of single factor experiment, content of steviol glycosides was determined with the authrone colorimetric method. The results showed that the best extraction condition was as follows, solid-liquid ratio 1︰60(g/m L), extraction time 60 min, ultrasonic power 150 W, temperature of 55 ℃, and extraction times as 3, and extraction rate was 27.32%.

关 键 词：甜叶菊 甜菊糖苷 超声波法 正交试验 蒽酮比色法 

分 类 号：TS201.2[轻工技术与工程—食品科学]