基于SPME-GC-MS与PCA的不同萃取头萃取刺梨汁香气成分效果比较  

Evaluation of aroma components in Rosa roxburghii Tratt juice by SPME-GC-MS and PCA

作　　者：顾苑婷 彭邦远 丁筑红 GU Yuan-ting;PENG Bang-yuan;DING Zhu-hong(School of Liquor and Food Engineering,Guizhou University,Guiyang,Guizhou 550025,China)

机构地区：[1]贵州大学酿酒与食品工程学院,贵州贵阳550025

出　　处：《食品与机械》2019年第1期47-53,共7页Food and Machinery

基　　金：贵州省教育厅自然科学研究项目(编号:黔教合KY字[2015366]号);贵州省科技重大专项(编号:黔科合重大专项字[2013]6006)

摘　　要：为提高刺梨汁香气成分气相色谱分析的准确可靠性,比较不同纤维涂层萃取头萃取刺梨汁香气成分的效果及灵敏度。采用50/30μm DVB/CAR/PDMS、75μm CAR/PDMS、65μm PDMS/DVB、100μm PDMS 4种萃取头进行刺梨汁香气成分的富集和浓缩处理,气质联用仪检测其香气成分,通过主成分分析法,对其香气成分的数量、含量进行比较,建立香气品质评价模型。结果表明,刺梨汁香气成分以酯类、醇类、萜烯类为主,不同萃取头萃取的化合物存在明显差异,50/30μm DVB/CAR/PDMS萃取化合物59种,相对含量最多为93.96%,其中酯类、醇类呈香物质含量最高;65μm PDMS/DVB萃取出38种化合物,相对含量为91.19%;100μm PDMS萃取出29种化合物,相对含量92.79%;75μm CAR/PDMS萃取出26种化合物,相对含量92.08%。在4种纤维涂层萃取头中,以50/30μm DVB/CAR/PDMS为富集刺梨汁香气成分的最佳萃取纤维。We used 50/30μm DVB/CAR/PDMS,75μm CAR/PDMS,65μm PDMS/DVB,100μm PDMS to enrich and concentrate aroma components of Rosa roxburghii Tratt juice for improving the accuracy and reliability of analyzing the aroma components by gas chromatography and comparing the consequence and sensitivity of extracting the aroma components by dif-ferent fiber coating extractors.The aroma components of Rosa roxburghii Tratt juice were detected by GC-MS.We compared the quantity and content of aroma components by principal component analysis(PCA)and established the evaluation model of aroma quality.The results showed that:esters,alcohols,and terpenes were the main aroma components of Rosa roxburghii Tratt juice.There were significant differences in the compounds extracted by different extraction heads.Fifty-nine compounds were extracted by 50/30μm DVB/CAR/PDMS with a relative content up to 93.96%.The contents of esters and alcohols were highest in aromatic materials.Thirty-eight compounds were extracted by 65μm PDMS/DVB with a relative content of 91.19%;twentytwo compounds were extracted by 100μm PDMS with a relative content of 92.79%;twenty-six compounds were extracted by 75μm CAR/PDMS with a relative content of 92.08%.Among the four fiber coating extraction heads,50/30μm DVB/CAR/PDMS was the best extraction fiber for the aroma components of the Rosa roxburghii Tratt juice.

关 键 词：刺梨汁 顶空固相微萃取 萃取纤维 香气成分 主成分分析 

分 类 号：TS255.44[轻工技术与工程—农产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程]