胶束电动毛细管色谱法分析3种交链孢毒素主成分含量  

Analysis of the main component of 3 kinds of alternaria toxins by micellar electrokinetic chromatographic

作　　者：胡佳 张晶[2,3] 邵兵 丁晓静[1,2,3] HU Jia;ZHANG Jing;SHAO Bing;DING Xiao-Jing(School of Public Health,Capital Medical University,Beijing 100069,China;Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning,Beijing Center for Disease Prevention and Control,Beijing 100013,China;China Research Center for Preventive Medicine,Beijing 100013,China)

机构地区：[1]首都医科大学公共卫生学院,北京100069 [2]北京市疾病预防控制中心食物中毒溯源技术北京市重点实验室,北京100013 [3]中国预防医学研究中心,北京100013

出　　处：《食品安全质量检测学报》2019年第6期1673-1678,共6页Journal of Food Safety and Quality

基　　金：重点领域急需化学成分量标准物质研制项目(2016YFF0201106)~~

摘　　要：目的 建立胶束电动毛细管色谱法(micellar electrokinetic chromatographic, MEKC)测定3种交链孢毒素[交链孢酚(alternariol, AOH)、交链孢酚单甲醚(alternariol monomethyl ether, AME)和交链孢菌酮酸(tenuazonic acid, TeA)]主成分含量的新分析方法。方法 以未涂层熔融石英毛细管柱(50μm×30 cm, 20 cm, i.d.)为分离柱,分别以3种分离缓冲溶液[40 mmol/L Na_2B_4O_7-120 mmol/L十二烷基硫酸钠(sodium dodecyl sulfate, SDS)(含体积分数为5%的乙腈)、30mmol/LNa_2B_4O_7-40mmol/LSDS(含体积分数为30%的异丙醇)和60mmol/L Na_2B_4O_7-180 mmol/L脱氧胆酸钠]及2种样品缓冲溶液[8 mmol/L硼砂(含体积分数为57%的甲醇用于AOH和TeA)、8mmol/L硼砂(含体积分数为57%的异丙醇用于AME)]分析AOH、AME和TeA的主成分含量。结果 AOH、AME、TeA的方法精密度分别为0.05%、0.01%和0.21%(n=5),检出限分别为5、15和0.5 mg/L。主成分含量分别为99.94%、99.78%和97.28%,且与高效液相色谱法(high performance liquid chromatography, HPLC)的结果无显著差异(α=0.05)。结论 MEKC法可作为HPLC法测定3种交链孢毒素主成分含量方法的互补方法 ,能够满足3种交链孢菌毒素主成分含量分析的要求。Objective To establish a new method for the main component analysis of alternariol(AOH),alternariol monomethyl ether(AME) and tenuazonic acid(TeA) by micellar electrokinetic chromatographic(MEKC).Methods An uncoated fused-silica capillary(50 μm×30 cm, 20 cm) was used for separation. Three kinds of separation buffer [40 mmol/L Na2B4O7-120 mmol/L SDS containing 5%(V/V) acetonitrile, 30 mmol/L Na2B4O7-40 mmol/L SDS containing 30%(V/V) isopropanol and 60 mmol/L Na2B4O7-180 mmol/L sodium doexycholate]combining with 2 kinds of sample buffer [8 mmol/L Na2B4O7 containing 57%(V/V) methanol for AOH and TeA,8 mmol/L Na2B4O7 containing 57%(V/V) isopropanol for AME] were used for main component analysis of 3 kinds of alternaria toxins. Results The precision of the method of AOH, AME and TeA was 0.05%, 0.01% and 0.21%(n=5),respectively. The limit of detection was 5, 15 and 0.5 mg/L, respectively. The main components of the 3 kinds of alternaria toxins were 99.94%、99.78% and 97.28%, respectively. There was no significant difference between the 2 methods of MEKC and high performance liquid chromatography(HPLC)(α=0.05). Conclusion The established MEKC is an alternative method to the HPLC, which can meet the requirements for the main component analysis of the above 3 kinds of toxins.

关 键 词：胶束电动毛细管色谱法 交链孢毒素 交链孢酚 交链孢酚单甲醚 交链孢菌酮酸 

分 类 号：TS201.6[轻工技术与工程—食品科学]