超声-乙醇法提取铁皮石斛花总黄酮及其体外抗氧化性的研究  

Extraction of total flavonoids from Dendrobium officinale flowers by ultrasonic-ethanol synergistic and its antioxidant activity

作　　者：缪园欣 廖明星 孙爱红[1] 丁文文 MIAO Yuanxin;LIAO Mingxing;SUN Aihong;DING Wenwen(School of Biological Engineering,Jingchu University of Technology,Jingmen 448000,China;Animal Husbandry and Veterinary Bureau of Jingmen City,Jingmen 448000,China;School of Medical,Jingchu University of Technology,Jingmen 448000,China)

机构地区：[1]荆楚理工学院生物工程学院,湖北荆门448000 [2]湖北省荆门市畜牧兽医局,湖北荆门448000 [3]荆楚理工学院医学院,湖北荆门448000

出　　处：《中国酿造》2019年第4期155-159,共5页China Brewing

基　　金：2018年湖北省知识创新专项(自然科学基金)(2018CFB305);荆楚理工学院引进人才科研启动金项目(QDB201607;QDB201708);荆楚理工学院优势特色学科群科研能力建设项目(17010305)

摘　　要：以铁皮石斛花为材料,通过单因素和正交试验对超声-乙醇法提取铁皮石斛花总黄酮的工艺进行研究。结果表明,超声波乙醇法提取铁皮石斛花总黄酮的最佳工艺为乙醇体积分数80%,料液比1∶35(g∶m L),超声温度40℃,超声时间50 min,在此条件下石斛花总黄酮提取率最高,为2.31%。对超声-乙醇法提取的铁皮石斛花总黄酮进行抗氧化的活性测定,试验结果显示铁皮石斛花总黄酮对DPPH·、·OH有明显的清除作用,当质量浓度达10 mg/m L时,其清除率分别为89.77%和80.01%。Using Dendrobium officinale flowers as raw materials,the extraction process of total flavonoids from D.officinale flowers with ultrasonic-ethanol synergistic extraction was studied by single factor and orthogonal experiments.The results showed that the optimum process parameters were ethanol concentration 80%,solid-liquid ratio 1∶35(g∶ml),ultrasonic temperature 40℃,time 50 min.Under the above conditions,the extraction rate of total flavonoids from D.officinale flowers was the highest of 2.31%.The antioxidant activity of the total flavonoids from D.officinale flowers were studied,and the total flavonoids had better scavenging ability on DPPH·and·OH.When the concentration was 10 mg/ml,the scavenging rate of DPPH·and·OH were 89.77%and 80.01%,respectively.

关 键 词：铁皮石斛花 总黄酮 超声波 乙醇 抗氧化性 

分 类 号：O652.62[理学—分析化学]