3,5-二硝基水杨酸法测定黄水中总糖的含量  

Determination of Total Sugar Content in Yellow Water by 3,5-Dinitrosalicylic Acid Method

作　　者：宋娇娇[1] 裴斐[1] 马勇 朱青 赵祥 SONG Jiaojiao;PEI Fei;MAYong;ZHU Qing;ZHAO Xiang(Yanghe Distillery Co. Ltd.,Suqian,Jiangsu 223800,China)

机构地区：[1]江苏洋河酒厂股份有限公司,江苏宿迁223800

出　　处：《酿酒科技》2019年第4期113-117,共5页Liquor-Making Science & Technology

摘　　要：建立3,5-二硝基水杨酸(DNS)测定黄水中总糖含量的方法,对检测条件和黄水总糖水解条件进行优化,并进行方法学考查。结果表明,最佳检测条件:显色剂添加量5 mL,显色时间7 min,显色温度90℃;黄水总糖水解条件为:水解时间30 min,盐酸浓度12 mol/L,盐酸添加量15 mL;精密度、重复性和稳定性试验结果的相对标准偏差(RSD)均小于2%,加标回收率94.60%～97.60%,RSD值均小于2%。该方法准确、可靠、重现性好,可用于黄水中总糖含量的测定。The determination of total sugar content in yellow water by 3,5- dinitrosalicylic acid (DNS) method had been developed. The optimum hydrolysis conditions of total sugar were summed up as follows: hydrolysis time was 30 min, hydrochloric acid concentration was 12 mol/L, and the addition level of hydrochloric acid was 15 mL. The optimum detection conditions were summed up as follows: DNS amount was 5 mL, coloration time was 7 min, and reaction temperature was at 90 ℃. The relative standard deviation (RSD) of precision, repeatability and stability were less than 2 %, the adding standard recovery rate were 94.60 % to 97.60 %, and RSD was below 2 %. This method was reliable, accurate and reproducible, and it could be used for the determination of total sugar content in yellow water.

关 键 词：3 5-二硝基水杨酸法 黄水 总糖 测定 

分 类 号：TS262.3[轻工技术与工程—发酵工程] TS261.7[轻工技术与工程—食品科学与工程]