莲子皮渣中酚类物质的提取优化及抗氧化活性评价  

Extraction Optimization and Antioxidant Activity of Phenolic Compounds from Lotus Seed Epicarp Residue

作　　者：蒋蕾 林美珠 茹巧美[2] 王淑萍 黄皓[1] 罗自生[1] JIANG Lei;Jarukitt Limwachiranon;RU Qiao-mei;WANG Shu-ping;HUANG Hao;LUO Zi-sheng(College of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou 310058,China;Hangzhou Wanxiang polytechnic,Hangzhou 310023,China)

机构地区：[1]浙江大学生物系统工程与食品科学学院,浙江杭州310058 [2]杭州万向职业技术学院,浙江杭州310023

出　　处：《现代食品科技》2019年第4期198-208,263,共12页Modern Food Science and Technology

基　　金：浙江省重点研发项目(2018C02049);杭州市农业科研攻关专项(20191203B31)

摘　　要：本文以莲子加工副产物-莲子皮渣为实验材料,在单因素实验的基础上,以总酚、总原花色素、DPPH·+清除能力为响应值,通过响应面设计优化莲子皮渣活性成分超声辅助提取条件,建立二次响应面回归模型,模型均显著且拟合性好。最优提取工艺为:丙酮浓度70%,料液比1:25.5 g/mL,提取时间1.6 h,提取温度50℃。该提取条件下,里叶白莲莲子皮渣提取物总酚、总原花色素、DPPH·+清除能力分别为194.54±3.63 mg GAE/g DW,243.15±2.88 mg CE/g DW,238.53±3.07 mg AAE/g DW,与预测值相符,证明实验建立的响应面模型可用于莲子皮渣中总酚、总原花色素、DPPH·+清除能力实验结果预测。对不同品种莲子皮渣植物化学素含量及体外抗氧化活性进行测定,发现莲子皮渣均富含多酚、类黄酮、原花色素、三萜等生物活性物质,且具有较高体外抗氧化活性。研究表明莲子皮渣富含生物活性成分,具有开发利用的巨大潜在价值。Lotus seed processing by-product-lotus seed epicarp residue was collected as experimental materials.Based on the results of single factor experiment,response surface methodology (RSM) was used to optimize the ultrasonic-assisted extraction of bioactive compounds from lotus seed epicarp residue,and total phenolic content (TPC),total proanthocyanidin content and DPPH·^+ scavenging capacity were set as response values.Experimental data were fitted to three quadratic polynomial models and the regression equations were significant with good fitting degree.The optimal extraction conditions were as follows:Acetone concentration of 70%,liquid to solid ratio of 1:25.5 g/ mL,extraction temperature as 50 ℃ and sonication time as1.6 hour.Under these conditions,the practical values of responses were 194.54±3.63 mg GAE/g DW, 243.15±2.88 mg CE/g DW,238.53±3.07 mg AAE/g DW,respectively,which were consistent with the predicted values.So the established models could accurately predict the results of total phenolic content,total proanthocyanidin content and DPPH·^+ scavenging capacity in lotus seed epicarp residue.We determined the phytochemical content and in vitro antioxidant activities of diverse lotus seed epicarp residue and found that they are rich in bioactive compounds including polyphenols,flavonoids,proanthocyanidins,triterpenes,and with high antioxidant activity. The study suggested that lotus seed epicarp residue contains high amount of bioactive chemicals and is of great potential for research and development.

关 键 词：莲子皮渣 多酚 原花色素 抗氧化 响应面 优化 

分 类 号：TS209[轻工技术与工程—食品科学]