SDE-GC-MS法重现性及普洱茶发酵香气变化研究  

Study on Reproducibility of SDE-GC-MS Method and Variation of Aroma Substances in Fermented Pu-erh Tea

作　　者：王利妍 郭天杰 石兴云 蒋宾 念波 焦文文 王旭东 赵明[1] 马燕[1] WANG Li-yan;GUO Tian-jie;SHI Xing-yun;JIANG Bin;NIAN Bo;JIAO Wen-wen;WANG Xu-dong;ZHAO Ming;MA Yan(College of Longrun Pu-erh Tea,Yunnan Agricultural University,Yunnan Kunming 650201,China)

机构地区：[1]云南农业大学龙润普洱茶学院,云南昆明650201

出　　处：《西南农业学报》2019年第4期810-815,共6页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31560221;31760225);国家农业产业技术体系茶叶专项(CARS-23);云南省中青年学术技术带头人后备人才培养项目(2017HB026)

摘　　要：【目的】为研究同时蒸馏萃取法提取普洱茶香气物质的重现性。【方法】以2次普洱茶发酵的原料(晒青毛茶)和出堆样为材料,SDE各提取3次,应用气相色谱质谱联用仪检测。【结果】各样品中鉴定出64～82个化合物,物质鉴定重复率为50.78%～71.30%;同一样品3次重复检测物质峰面积的精密度(RSD)为0.76%～70.91%。原料以醇类物质(53.3%和50.0%)为主,发酵后1,2,3-三甲氧基苯、3,4,5-三甲氧基苯等杂氧化合物增加,出堆样以杂氧化合物(44. 0%和41.5%)为主。【结论】SDE-GC-MS法研究茶叶香气需增加重复次数或改进方法。【Objective】The aroma substances in Pu-erh tea were studied by Simultaneous-dist extraction(SDE)previously,however,the reproducibility of these methods were not studied.【Method】In this work,two raw materials and two Pu-erh tea fermented samples were extracted three times by Simultaneous-dist extraction method(SDE),and detected by Gas Chromatography-mass Spectrometry(GC-MS),respectively.【Result】The 64-82 compounds were detected,and the repetition rate of substance identification were 50.78%-71.30%.The reproducibility(RSD)of peak areas detected in the same samples for three times was 0.76%-70.91%.The main aroma substances in raw materials(R1 and R2)were alcohol with relative contents of 53.3%and 50.0%,respectively.After fermentation,relative levels of heterocyclic compounds,such as 1,2,3-trimethoxy benzene and 3,4,5-trimethoxy benzene were increased.And main aroma substances in fermented samples(P1 and P2)were heterocyclic compounds with relative contents of 44.0%and 41.5%,respectively.【Conclusion】It need to develop more repeats analyses or improve the extraction method in the study of aroma substances of Pu-erh tea using SDE-GC-MS method.

关 键 词：普洱茶 香气物质 重现性 气相色谱质谱仪 同时蒸馏萃取法 

分 类 号：S571[农业科学—作物学]