橙子果皮诱导抗病组分对意大利青霉的抑菌活性及作用机制  

Antifungal Activity and Possible Mode of Action of Induced Disease-Resistant Components in Orange Peels against Penicillium italicum

作　　者：彭洋 杨书珍[1] 张美红 程运江[2] 彭丽桃[1] PENG Yang;YANG Shuzhen;ZHANG Meihong;CHENG Yunjiang;PENG Litao(College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070,China;College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070,China)

机构地区：[1]华中农业大学食品科技学院,湖北武汉430070 [2]华中农业大学园艺林学学院,湖北武汉430070

出　　处：《食品科学》2019年第9期1-6,共6页Food Science

基　　金：国家自然科学基金青年科学基金项目(31471633)

摘　　要：对前期研究发现的毛霉诱导脐橙果皮产生的抗病性红色物质组分进行分离纯化,并分析其对柑橘意大利青霉的抑菌活性及作用机制。结果表明,在50～200μg/m L质量浓度下,红色物质组分对意大利青霉孢子萌发、芽管伸长、菌落生长和菌丝生物量增加表现出很强的剂量依赖型抑制作用。进一步研究表明:红色物质处理改变了菌丝细胞壁几丁质结构与分布,降低了几丁质含量;破坏了细胞膜通透性,降低了细胞膜总脂质含量和麦角固醇含量,影响了细胞膜的正常功能。说明红色物质作为替代化学杀菌剂的新型柑橘采后安全保鲜剂具有良好的研究和开发前景。In our previous study, we demonstrated that inoculation of orange peels with Actinomucor elegan could induce the accumulation of disease-resistant components, red in color. Here, the red pigments were collected and purified to evaluate their antifungal activity against Penicillium italicum and the possible mode of action was investigated. The results showed that at concentrations of 50–200 μg/mL, the red pigments exhibited strong antifungal activity in a dose-dependent manner as demonstrated by the ability to inhibit spore germination, germ tube elongation, mycelial growth and biomass accumulation. Furthermore, the red pigments altered cell wall structure and chitin distribution, and reduced the content of chitin in the cell wall. They destroyed cell membrane permeability and decreased ergosterol and total lipid contents in the cell membrane, thereby affecting the normal function of the cell membrane. Our results demonstrated that the red pigments can be used as a promising natural alternative to chemical fungicides for controlling postharvest diseases of citrus fruits.

关 键 词：抗病性组分 抑菌活性 抑菌机理 细胞膜 细胞壁 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]