黄酒通过肠肌间神经丛抑制大鼠离体小肠的收缩  

Chinese Rice Wine Inhibits Contraction Activity of the Isolated Rat Small Intestine through Intestinal Myenteric Plexus

作　　者：王聪 赵盼 周亚男 王天霞 孙海基[1] WANG Cong;ZHAO Pan;ZHOU Yanan;WANG Tianxia;SUN Haiji(School of Life Science, Shandong Normal University, Jinan 250014,China)

机构地区：[1]山东师范大学生命科学学院,山东济南250014

出　　处：《食品科学》2019年第9期173-178,共6页Food Science

基　　金：公益性行业(农业)科研专项(201303069-07);国家自然科学基金面上项目(31672286)

摘　　要：目的:观察不同剂量的黄酒对大鼠离体小肠平滑肌收缩的影响并探究其可能的机制。方法:用低、中、高剂量的黄酒,乙醇,去多酚黄酒分别刺激大鼠离体小肠,RM6240多道生理信号采集系统记录大鼠离体小肠各节段加药前1 min和加药后第2分钟的收缩曲线,用肠道全层铺片免疫组织化学和Western blot技术检测大鼠肠肌间神经丛乙酰胆碱转移酶(choline acetyltransferase,ChAT)和一氧化氮合成酶(nitric oxide synthase,NOS)的表达。结果:与黄酒处理前相比较,中、高剂量(黄酒体积分数分别为3.5%、10.0%)的黄酒能明显抑制大鼠离体小肠的收缩幅度,抑制率分别为58%和100%;而中剂量的乙醇对离体小肠的收缩无明显的影响,高剂量的乙醇能显著抑制离体小肠的收缩幅度(抑制率为47.5%)。与处理前比较,经中、高剂量去多酚黄酒处理后,离体小肠运动幅度明显降低(P<0.05),抑制率分别为48%和67%。黄酒组肠肌间神经丛ChAT蛋白的表达量显著减少,而NOS蛋白的表达量显著增加。结论:黄酒对离体小肠收缩有明显的抑制作用,其作用与黄酒中的乙醇和多酚成分有关。黄酒可能通过抑制肌间神经丛中兴奋性神经递质乙酰胆碱的释放,促进抑制性神经递质一氧化氮的释放,而导致离体小肠的收缩受到抑制。黄酒能通过影响肌间神经丛内神经元的活性,抑制小肠运动,减缓胃肠机能亢进,为黄酒的保健功能研究及推广提供了理论依据。Objective: The aim is to explore the effect and mechanism of Chinese rice wine(CRW) on the contraction activity of the isolated small intestine of rats. Methods: The isolated small intestine was treated separately with low, medium, high concentrations of CRW, alcohol and CRW without polyphenols. A RM6240 multi-channel physiological signal acquisition system was used to record intestinal motility. The protein expression of choline acetyltransferase(ChAT) and nitric oxide synthase(NOS) in intestinal myenteric plexus was measured by immunohistochemistry and Western blot. Results:Compared with the control group, medium and high-dose CRW(3.5% and 10.0%, V/V) obviously inhibited the amplitude of intestinal contraction with percentage inhibition of 58% and 100%, respectively. Medium-dose ethanol had no significant impact on intestinal contraction, but high-dose ethanol significantly inhibited the amplitude of intestinal contraction with a percentage inhibition of 47.5%. Medium and high-dose CRW without polyphenols significantly inhibited the contractile amplitude of the isolated small intestine by 48% and 67%, respectively, but the efficacy was inferior to that of CRW.Compared with the control group, the expression of ChAT in intestinal myenteric plexus in the CRW group significantly decreased, while the expression of NOS significantly increased. Conclusion: The effect of CRW on contraction of isolated small intestine is related to its ingredient ethanol and polyphenols, by inhibiting the release of acetylcholine(ACh) as an excitatory neurotransmitter and enhancing the release of NO as an inhibitory neurotransmitter from intestinal myenteric plexus. CRW can affect the activity of neurons in the myenteric plexus, inhibit the movement of small intestine, and slow down the hyperactivity of gastrointestinal function, which will provide new clues to the health-benefiting effects of CRW.

关 键 词：黄酒 小肠运动 肌间神经丛 乙酰胆碱 一氧化氮 

分 类 号：R151.1[医药卫生—营养与食品卫生学]