D-松醇复配Mn^(2+)对HepG2细胞胰岛素抵抗的调节及其作用机制  

Effects and Mechanism of D-pinitol Compound with Mn^(2+) on Insulin Resistance of HepG2 Cells

作　　者：王梦 张泽生[1] 李雨蒙[1] 刘亚萍 张文菱子 WANG Meng;ZHANG Ze-sheng;LI Yu-meng;LIU Ya-ping;ZHANG Wen-ling-zi(College of Food Engineering and Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China)

机构地区：[1]天津科技大学食品工程与生物技术学院,天津300457

出　　处：《食品工业科技》2019年第10期139-144,共6页Science and Technology of Food Industry

摘　　要：采用胰岛素诱导HepG2细胞建立胰岛素抵抗模型(IR),研究D-松醇复配Mn2+对细胞葡萄糖消耗量和糖原合成量的影响。实时荧光全定量分析(RT-PCR)实验检测AMPK信号通路相关基因mRNA表达水平。结果表明当胰岛素浓度为1×10^(-3)mmol/L,作用时间为36 h时,细胞葡萄糖消耗量达到最低,产生最大的胰岛素抵抗效应。与模型对照组相比,当D-松醇浓度为100 mg/L,复配MnSO4为10 mg/L时,葡萄糖消耗量和糖原含量均极显著升高(p <0.01)。此外,复配组AMPKα-1、AMPKα-2和GLUT4基因表达水平均显著上调(p <0.05),G6Pase基因mRNA表达水平显著下调(p <0.05)。D松醇复配Mn^(2+)可显著促进胰岛素抵抗细胞葡萄糖的利用,增加糖原合成,其作用机制可能涉及AMPK参与的抑制糖异生等生化调控过程起到降血糖作用。The insulin resistant models of HepG2 cells were induced by insulin to study the effects of D-pinitol compound with Mn^2+ on glucose consumption and glycogen synthesis.The expression of AMPK genes was detected with real-time polymerase chain reaction( RT-PCR). The results showed that when the insulin concentration was 1 × 10^-3 mmol/L and the action time was 36 h,the glucose consumption reached the minimum.Compared with the model group,when the D-pinitol concentration was100 mg/L and MnSO4 was 10 mg/L,glucose consumption and glycogen content were extremely significantly( p < 0.01). The mRNA expression levels of AMPKα-1,AMPKα-2 and GLUT4 increased significantly( p < 0.05) while G6 pase mRNA expression decreased significantly( p < 0.05). D-pinitol compound with Mn^2+ could improve the glucose consumption,increase glycogen synthesis.Moreover,it had a positive effect on hypoglycemic activity through AMPK signaling pathway.

关 键 词：D-松醇 复配 MN^2+ HEPG2细胞 AMPK 

分 类 号：TS201.1[轻工技术与工程—食品科学]