Effect of broad-spectrum antibiotics on bacterial translocation in burned or septic rats  

作　　者：Zhen-Liang Wen Li-Di Zhang Shao-Ze Liu Jiao Liu Yi-Zhu Chen De-Chang Chen 

机构地区：[1]Department of Critical Care Medicine, Ruijin Hospital North, Shanghai Jiao Tong University School of Medicine, Shanghai 201801, China [2]Department of Emergency Center, the 281 Hospital of Chinese People's Liberation Army, Qinhuangdao, Hebei 066100, China

出　　处：《Chinese Medical Journal》2019年第10期1179-1187,共9页中华医学杂志（英文版）

基　　金：grants from the National Natural Science Foundations of China (No. 81571943 and No. 81272137).

摘　　要：Background: Antibiotics are frequently used to treat critically ill patients, and its use is often accompanied by intestinal dysbiosis might further lead to bacterial translocation (BT). Nevertheless, studies on the relationship between antibiotic therapy and BT are rare. In the present study, we investigated the effect of broad-spectrum antibiotics on BT in an experimental rat model of burn or sepsis injury. Methods: The septic rat model was simulated by a second insult with lipopolysaccharides after burn injury. Ninety-two male Sprague-Dawley rats were randomly divided into control, burn, and sepsis groups (n = 8 or 9, each group), and the latter two groups were then treated with imipenem or ceftriaxone for 3 or 9 days. The mesenteric lymph nodes, liver, lungs, and blood were collected at each time point under sterile conditions for quantitative bacterial culture and strain identification. The differences between the groups were compared by Fisher exact test or Mann-Whitney U test. Results: Only minimal Escherichia coli translocation to the mesenteric lymph nodes was observed in the normal control group, in which the BT rate was 12.5%. Burn injury did not affect the BT rate (Burn group vs. Control group, 12.5% vs. 12.5%, P = 1.000), whereas the BT rate showed an increased trend after the second insult with lipopolysaccharide (Sepsis group us. Control group, 44.4% 12.5%, P = 0.294), and many strains of Enterobacteria spp. were detected in distant organs (liver, lung, and blood)[Sepsis group vs. Control group, 0 (0,3) us. 0 (0,0), U = 20, P = 0.045]. After the antibiotic treatment, BT to the distant organs was increased in burned rats [Burn IT3 group us. Burn group, 0 (0,2) vs. 0 (0,0);Burn IT9 group vs. Burn group, 0 (0,1) vs. 0 (0,0);Burn CT9 group Burn group, 0 (0,2) vs. 0 (0,0);all U = 20 and P = 0.076] but decreased in septic rats [Sepsis CT3 group vs. Sepsis group, 0 (0,0) vs. 0 (0,3), U = 20, P = 0.045]. The total amount of translocated bacteria, regardless of which antibiotic was used, was increased in bu

关 键 词：Antibiotic BACTERIAL TRANSLOCATION ENTEROCOCCI ENTEROBACTERIA SEPSIS 

分 类 号：R[医药卫生]