青钱柳黄酮及三萜调节人体肠道菌群作用研究  

Modulatory Effect of Flavonoids and Triterpenoids from Cyclocarya paliurus on Human Intestinal Microbiota

作　　者：陈玉慧 郭彤彤 张鑫[1] 郑晓杰[2] Chen Yuhui;Guo Tongtong;Zhang Xin;Zheng Xiaojie(School of Marine Sciences,Ningbo University,Ningbo 315211,China;Department of Agriculture and Biotechnology,Wenzhou Vocational College of Science and Technology,Wenzhou 325000 China)

机构地区：[1]宁波大学海洋学院,浙江宁波315211 [2]温州科技职业学院农业与生物技术学院,浙江温州325000

出　　处：《中国野生植物资源》2019年第2期6-10,15,共6页Chinese Wild Plant Resources

基　　金：浙江省重点研发项目(2017C02039)

摘　　要：以青钱柳为材料,通过热水浸提,大孔树脂柱层析分离纯化,制备得到纯度大于90%的青钱柳黄酮和三萜。继而采用体外厌氧粪样混合培养与荧光原位杂交技术,评价青钱柳黄酮和三萜对于人体肠道菌群的调节作用。结果表明青钱柳黄酮和三萜类物质能有效促进肠道中有益细菌(双歧杆菌和乳酸菌)的增殖,而对于梭状菌和拟杆菌则有抑制作用,同时未显著影响肠道总菌群的数量。研究表明,青钱柳中的黄酮和三萜类物质,对于改善人体肠道环境、维护人体健康具有重要作用。In the present study,high purity(>90%)of flavonoids and triterpenoids were prepared from Cyclocarya paliurus by extraction with hot water and simple purification procedures of polyamide and AB-8 column chromatography.Then the modulatory effect of flavonoids and triterpenoids on human intestinal microbiota was evaluated through anaerobic mixed culture of fecal samples,and the bacterial populations were determined by fluorescent in situ hybridization(FISH).It can see that Cyclocarya paliurus flavonoids and triterpenoids promoted the growth of the beneficial bacteria(Bifidobacterium spp.and Lactobacillus/Enterococcus spp.),showed the inhibitory effect on Bacteroids-Prevotella spp.and Clostridium histolyticum group,while they did not affect the population of total bacteria.It can be inferred that Cyclocarya paliurus flavonoids and triterpenoids have certain regulation effects on intestinal environment by the modulation of the intestinal bacterial populations,contributing to the improvements of host health.

关 键 词：青钱柳 三萜 黄酮 肠道菌群 

分 类 号：TS272[农业科学—茶叶生产加工]