低聚糖和多酚结合偏最小二乘判别分析鉴别油菜蜜产地  

Discrimination of Geographical Origins of Rape Honey by Polyphenol and Oligosaccharide Measurements Combined with Partial Least Square-Discrimination Analysis

作　　者：佘僧 李熠[1,3] 宋洪波 陈兰珍[1,3] SHE Seng;LI Yi;SONG Hongbo;CHEN Lanzhen(Institute of Apicultural Research,Chinese Academy of Agricultural Sciences,Beijing 100093,China;College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Laboratory of Quality and Safety Risk Assessment for Bee Products (Beijing),Ministry of Agriculture and Rural Affairs,Beijing 100093,China)

机构地区：[1]中国农业科学院蜜蜂研究所,北京100093 [2]福建农林大学食品科学学院,福建福州350002 [3]农业农村部蜂产品质量安全风险评估实验室(北京),北京100093

出　　处：《食品科学》2019年第12期290-295,共6页Food Science

基　　金：国家自然科学基金面上项目(31772070);中国农业科学院创新工程项目(CAAS-ASTIP-2017-IAR);现代农业(蜂)产业技术体系专项(CARS-45-KXJ10)

摘　　要：采用气相色谱-串联质谱技术测定油菜蜜中6 种低聚糖成分,高效液相色谱-串联质谱技术测定油菜蜜中18种酚酸物质,并结合偏最小二乘判别分析(partial least square-discrimination analysis,PLS-DA)对来自湖北钟祥市、江苏盐城市、青海刚察县3个具有显著地理、气候、环境差异的51 个油菜蜜样本进行产地鉴别。方差分析结果显示:3个产地油菜蜜中松二糖含量具有显著性差异,且青海刚察油菜蜜低聚糖含量相对偏高;油菜蜜的18种多酚类物质中大多数具有显著的地理差异性且湖北钟祥的油菜蜜中多酚含量相对偏高。多元统计分析结果显示多酚具有显著的地理特征性。油菜蜜中低聚糖和多酚的含量结合PLS-DA产地鉴别的预测精度可达到97%。In this study, 6 kinds of oligosaccharides in rape honey were determined by gas chromatography-mass spectrometry and 18 kinds of phenols by high-performance liquid chromatography-mass spectrometry (HPLC-MS). A new method was used to discriminate rape honey samples from different geographical origins through analysis of oligosaccharides and polyphenols combined with partial least squares discriminant analysis (PLS-DA). For this study, we collected 51 samples from Zhongxiang city of Hubei province, Yancheng city of Jiangsu province and Gangcha county of Qinghai province, among which there are signi ficant geographical, climatic and environmental differences. The results of analysis of variance (ANOVA) showed that there were significant differences in the turanose content of rape honey samples from the three regions, and the oligosaccharide content of rape honey form Gangcha was relatively higher. There were signi ficant geographical differences in the contents of most polyphenols and the polyphenols content of rape honey from Zhongxiang was relatively higher. Multivariate statistical analysis showed that polyphenols were a significant indicator for the geographical origin of honey, and the geographical origins of rape honey could be predicted with an accuracy of 97% by the contents of oligosaccharides and polyphenols combined with PLS-DA.

关 键 词：低聚糖 酚酸 油菜蜜 产地溯源 偏最小二乘判别分析(PLS-DA) 

分 类 号：S896.1[农业科学—特种经济动物饲养]