基于氧化还原电位的光合细菌补料培养工艺优化  

Feeding Culture Process Optimization of Photosynthetic Bacteria Based on Oxidation Reduction Potential

作　　者：王志华 朱晓雯 何桂霞 王泽建[1] 王永红[1] 庄英萍[1] WANG Zhi-hua;ZHU Xiao-wen;HE Gui-xia;WANG Ze-jian;WANG Yong-hong;ZHUANG Ying-ping(State Key Laboratory of Bioreactor Engineering,National Engineering Research Center for Biotechnology,East China University of Science and Technology,Shanghai 200237,China;Mingjin Shanghai Livestock Breeding Limited Company,Shanghai 202150,China)

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,国家生化工程技术研究中心,上海200237 [2]上海明锦畜牧养殖有限公司,上海202150

出　　处：《食品工业科技》2019年第12期141-144,148,共5页Science and Technology of Food Industry

基　　金：上海市科技兴农推广项目(沪农科推字[2016]第2-2-1号)

摘　　要：为了提高光合细菌培养密度,优化补料工艺,将在线氧化还原电位系统用于光合细菌培养过程中指导主要碳源琥珀酸钠的流加,并且考察琥珀酸钠不同补加浓度以及补料方式对菌体浓度的影响。结果表明,氧化还原电位(ORP)回升可以作为菌体因缺少主要碳源琥珀酸钠而停止增长的信号。相比于批培养,当补入琥珀酸钠速度为0.08 g·(L·h)-1时光合细菌生长达到最佳,细胞密度由0.611 g/L提高至0.927 g/L。而基于ORP指导的间歇碳源流加工艺优于连续流加,最终菌群培养密度可提高至1.46 g/L。基于氧化还原电位的光合细菌补料培养工艺大大提高了生产效益,为后续的工业化生产提供了一定的指导意义。In order to improve the culture density of photosynthetic bacteria and optimize the feeding process,the online redox potential system was used to guide the flow of the main carbon source sodium succinate during the photosynthetic bacteria culture process. The different supplemental concentrations of sodium succinate and the feeding mode were investigated. The results showed that oxidation reduetion potential(ORP)rebound could be used as a signal that the bacteria stopped growing due to the lack of the main carbon source sodium succinate. Compared with batch culture,photosynthetic bacteria grew optimally when the rate of sodium succinate was 0.08 g·(L·h)-1,and the cell density increased from 0.611 g/L to 0.927 g/L. The intermittent carbon source addition process based on ORP was superior to continuous flow addition,and the final microbial culture density could increased to 1.46 g/L. The feeding culture process of photosynthetic bacteria based on ORP greatly improved the production efficiency and provided certain guiding significance for the subsequent industrial production.

关 键 词：光合细菌 补料分批培养 氧化还原电位 工艺优化 

分 类 号：TS201.3[轻工技术与工程—食品科学]